Pick six “partial diploid” lac operon problems from the lac operon practice sheet.  (Those are the ones with 2 complete operons, i to t.) Figure out the answer.

A revertant has been isolated from the E. coli mutant. The original mutant contains a mutation A136 to G in gene Z. The gene sequencing of this revertant E. coli strain showed that gene Z still had the original mutation at A136 to G, but no other mutations in gene Z were found. Such a reversion is called _________________. Select one: a. silent suppression b. true reversion. c. intragenic suppression d. intergenic suppression.

i need help finding the right answer

Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following partial diploids for the lac operon strains.

What would be the transcriptional output of the lac operon in an E. coli cell in the following 8 scenarios? Your answers must be one word each, selected from the following two options: 'high' and 'low.

Can you give an example of a modified (tagged) primers to amplify E. coli UidA sequence and subclone into appropriate vector (pUC19)?

The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…

Operons A. Draw a schematic of the lac operon when glucose is low and lactose is high in the cell’s environment. B. Draw a schematic of the trp operon when tryptophan levels in the environment are low.  C. What is the lux operon and how is affected by quorum sensing?

Predict the growth/color pattern you'd expect for a mutant that can't activate both sugar operons (like the lac and mal operon). Table 1: Options are blue, white, or either for NA plates; Table 2: Options are: growth or none for minimal plates). Table 1 either Table 2 no growth Xgal glucose white Xgal + lactose growth Minimal lactose ΝΑ blue Xgal + IPTG growth maltose blu

can I have a summary explanation  what effect does lactose have on the bacterial cell’s lac operon?