1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particles

1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particles

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1TYK

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Part 1. Gene Cloning 1) How is a gene inserted into a plasmid cloning vector? Be specific, but be brief. No more than 2 (non-run-on) sentences please, and do not exceed this space. 2) A geneticist has transformed a vector with an ampicillin resistance gene into E. coli cells and plated those cells on media containing ampicillin. She comes back 24 hours later and sees only blue colonies on the media. a) How does she know that the cells growing on the media were successfully transformed with the vector? b) What does it mean that the colonies are blue?
The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1. T7 RNA polymerase 2. Reverse transcriptase 3. RNaseH
1. As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance gene and a lacZ gene, within which is located a multiple cloning site. Briefly describe how one selects for/identifies bacterial cells containing recombinant pBluescript II molecules and explain how the selection/identification works.
Describe how P1vir transduction can be used to introduce a gene mutation into E. coli USING DIAGRAMS ONLY. small figures to explain the diagrams is allowed. You should describe / show both the molecular biology and the transduction procedure
4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for the enzyme glutathione-S-transferase (GST) is used in purifying your protein of interest. Briefly explain how this is done. 6) What enzyme is used to create CDNA from MRNA?
Section two. Match horizontal gene transfer mechanism with best definition. Answers are used more than once. а. Transduction b. Transformation Conjugation c. 44. requires two live cells, where the recipient receives donor DNA 45. involves a virus 46. usually a plasmid is transferred 47. does not require a virus or another cell 48. "sex" for bacteria 49. occurs because of mistake made in chromos ome packaging by a virus 50. Frederick Griffith coined this term.
91) The NDV virus a. The mesogenic ND is less virulent than lentogenic than NDV b. The antigenomic copy is the template for both making genome and making subgenus c. The RNA polymerase is only made in the first round of translation d. The Fusion protein is the virulent protein
Which of the following apply to the development of urogenital tract Candida albicans infections? Disruption of population dynamics allowing the RB form of Chlamydia to cause tissue damage Loss of Lactobacillus populations leading to an increase in the local pH Loss of Gardnerella vaginalis populations leading to a lose of Clue cells Loss of the anaerobic environment that promotes the outgrowth of Trichomonas vaginalis None of the answers apply
18. You want to express hemoglobin beta (HBB) in a bacteria model using a vector. You would like to amplify the following gene by PCR. GTAGAATATG ATAAGCGAAA CTGCAAATCG CGTTTGGGGC GATACAAGTA GTGTACGCGG ACCGCGCCGA GCGGGCTATG GTTTCTCCAC TATCAGTTCT TCTCCTGTCC CAGCGAAGTC GAGGTCCAGC CCTACGGTAC ATAACTAACA CGGTTTGAAG AAGATACGAT CTTACGAAGT AAAGAAAATT TGTAGTCAGC CCGGTTCGCT TGTGTCCAGC TAATCGATTG ATTGGCCCCA GCAGGCGAGA TGAACATAGT CATGCGCTGT CTAATAGCCC ATTTGACGTG TAGGTGGCGC TTTTATTTCT GAGGTGGAAA T a) If the primers were both 20 nucleotides long, what are the sequences for both PCR primers that will amplify only the highlighted region? Be sure to label the 5' and 3' ends. (4 points) b) What is the length (in bp) of the entire region amplified by their primers (i.e. the amplicon length)? (Note: the spacing in the above sequence is intentionally uniform) (2 points) c) If you start with 300 copies template DNA how many copies would you expect to produce if you ran the PCR for 22 cycles? Show your work.…
Define the following terms:a. satellite DNAb. transpositionc. transposond. retrotransposone. endogenous retrovirus
1. Determine what is being meant by the statements: a. What is a binary vector? What characteristic does it have? b. What is the role of the vir genes in the Ti plasmid c. System for negative regulation of transcription of bacterial genes involved in lactose metabolism.
2) Transgenic animal refers to an organism whose genome has been altered by the transfer of a gene or genes from another species or breed. Cows, pigs and goats had been modified especially for pharmaceutical purposes. Explain three (3) most used transfection technology for the creation of transgenic animals.