B. Arrange the following steps in the genetic engineering of corn to acquire pest resistance. to indicate the correct sequence. The tumor-inducing plasmid will be incorporated into the genome of the infected corn cells. Write numbers 1 to 1O 1. 2. Insert the cry1Ab gene into the tumor-inducing plasmid of Agrobacterium 3. tumefaciens by using ligase. Treat the isolated genetic material with restriction enzymes and subject it to gel electrophoresis and probes to identify and isolate the cry1Ab gene. 4. Regenerate the cultured corn cells and allow them to grow into mature Bt corn. 5. Reintroduce the recombinant plasmid into Agrobacterium tumefaciens cells. 6. Allow the transformed Agrobacterium tumefaciens cells to infect the cultured corn cells. 7. Cross the mature Bt corn with other superior corn varieties. 8. Obtain and culture Bacillus thuringiensis cells as sources of the cry1Ab gene. 9. Determine the source of the gene of interest which expresses the pest resistance trait. 10. Isolate the genetic material from Bacillus thuringiensis. C. Answer the following question. 1. If you are able to successfully incorporate foreign DNA to your host organism, whatare your major concerns thereafter? 9 | MODULE NUMBER 1 THIRD QUARTER

B. Arrange the following steps in the genetic engineering of corn to acquire pest resistance. to indicate the correct sequence. The tumor-inducing plasmid will be incorporated into the genome of the infected corn cells. Write numbers 1 to 1O 1. 2. Insert the cry1Ab gene into the tumor-inducing plasmid of Agrobacterium 3. tumefaciens by using ligase. Treat the isolated genetic material with restriction enzymes and subject it to gel electrophoresis and probes to identify and isolate the cry1Ab gene. 4. Regenerate the cultured corn cells and allow them to grow into mature Bt corn. 5. Reintroduce the recombinant plasmid into Agrobacterium tumefaciens cells. 6. Allow the transformed Agrobacterium tumefaciens cells to infect the cultured corn cells. 7. Cross the mature Bt corn with other superior corn varieties. 8. Obtain and culture Bacillus thuringiensis cells as sources of the cry1Ab gene. 9. Determine the source of the gene of interest which expresses the pest resistance trait. 10. Isolate the genetic material from Bacillus thuringiensis. C. Answer the following question. 1. If you are able to successfully incorporate foreign DNA to your host organism, whatare your major concerns thereafter? 9 | MODULE NUMBER 1 THIRD QUARTER

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 7.3P

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