In 1973. Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacterium. The bacterium then began producing a proteir by the code found on the inserted frog gene. The procedure used by Cohen and Boyer created a... O A. Transgenic organism O B. Non-living organism O C. Stem cell O D. Unspecialized cell

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13) The pLacZ cloning vector has an MCS with 10 different palindromes, that is, where you can clone using 10 different enzymes. How many different combinations of enzymes could be used to clone? (This is a measure of the versatility of the vector) a) 2^10 b. 4^3 c. 10^2 d. 3^4 14)One of the first vaccines achieved thanks to the tissue culture technique was that of influenza, True or False? 15) In the context of biotechnology, what does the term clone mean? a) Synonym of Dolly, identical copies of a multicellular organism b. Many copies of some cellular organelle. c. Production of drugs and medicines for diseases, but under the control of ethical standards d. Chimeric molecules (vector and insert), inside a bacteria replicating to form a colony e. Identical copies of proteins from recombinant DNA

8. A mutant that has a nutritional requirement for growth is an example of a(n) A) heterotroph B) auxotroph C) autotroph D) organotroph 9. In the Ames test the number of histidine auxotroph colonies growing on media lacking histidine when exposed to a potential chemical mutagen is. proportional to the likelihood that the chemical is mutagenic. A) Directly B) Inversely 10. Thymine dimers result from A) ionizing radiation B) nucleoside analogs C) non-ionizing radiation D) none of the above 12. RecA proteins are NOT required for homologous recombination? A) True B) False 13. Increasing genetic variability by the uptake of DNA from the environment is A) transduction B) transformation C) a mutation D) conjugation 14. Which of the following features are common to transformation, transduction, and conjugation? (1) unidirectional transfer of genes (2) incomplete gene transfer (3) homologous recombination (4) meiosis occurring in the recipient A) 1, 2, 4 B) 1, 2, 3 C) 3,4 D) 1,2 15. What is…

Retrotransposons _______. A) are found only in animal cells B) use an RNA molecule as an intermediate in transposition C) generally move by a cut-and-paste mechanism

What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases?   a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d.   The DNA band detected by Southern blot is different from that by Northern blot

If a researcher accidentally transformed the bacterial two hybrid plasmids ( pKT25-X and pUT18-Y) into a normal strain of E coli that produces adenylate cyclase, his/her experiment will: a. show a high lacZ activity in all tubes (control and experiment) because Proteins X and Y do not need to be interacting in order to activate lacZ gene prescription. b. not work because cells lack cAMP. c. need replicates in order to show true interaction between X and Y.

You are studying celiacs disease and have identified a potential gene for the disease using linkage analysis. You decide to test your hypothesis by making transgenic mice that carry a nonsense mutation in the candidate gene you have identified to see if they develop a phenotype similar to that found in Celiacs disease What sort or experimental approach are you using when you test your gene in your mouse model? a. Reverse genetic b. Quantitative genetic C. Forward genetic D.Expression analysis E. None of the above

The 6th and 7th women Nobel Prize winners in Chemistry, Professors Jennifer Doudna and Emmanuelle Charpentier, developed what technology that utilizes DNA base pairing through their keto-like tautomeric forms? Your answer O a. CRISPR gene editing O b. Flexible display monitors O c. Smartphones O d. Fingerprinting

8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B. Used to test for the presence of hundreds to thousands of active genes at the same tim C. Used as a vector to replicate foreign DNA inside bacteria D. Able to cut double stranded DNA at almost any desired location for genome editing Which of the following is not an example of RNA processing. the addition of the poly-A tail to mRNA in the nucleus spliceosomes cutting mRNA in the nucleus the addition of the 3' cap to mRNA in the nucleus the joining together of exons in the nucleus