_4] Xylose gives a positive result with Molisch's, Seliwanoff's, & Bial's-Orcinol tests. 5] Aniline-acid oxalate can be use as a mobile phase for the development of the chromatogram. _6] Ethanol (95%) & 10% TCA are used to precipitate glycogen from the solution.
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![_4] Xylose gives a positive result with Molisch's, Seliwanoff's,
& Bial's-Orcinol tests.
5] Aniline-acid oxalate can be use as a mobile phase for the
development of the chromatogram.
_6] Ethanol (95%) & 10% TCA are used to precipitate
glycogen from the solution.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F80101996-1698-4c40-8ebf-c59495691253%2Fad27858b-b5f0-4170-ad5a-f44947693860%2Fsr1e4x_processed.png&w=3840&q=75)
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- I need to prepare a standard calibration curve for gamma globulin. absorbances on Y and mg of standard protein per assay on X. used 0.1mg/ml gamma stock for tubes 2-6. (Water (ml), gamma (ml), Abosrbance)--> (.036, .004, .290) (.036, .008, .358) (.024, .016, .341) (.016, .024, .520) (.008, .032, .597) - What is the math and how do you get the standard curve?Determine the concentration of L. monocytogenes enumerated from the apple slice based on the information below. You must draw your serial dilution and show your mathematical calculation. The apple slice (12 g) was first mixed with 108 ml of sterile 0.1% peptone water. After, 1 ml was added to tube containing 9 ml of sterile 0.1% peptone water. Process was repeated with two additional tubes. 1 ml was pipetted from tubes 2 and 3 onto MOX media. Plates were incubated at 37 for 48 hours. The pictures below show the plates from tube 2 (left) and tube 3 (right).Question 1: Suppose you have given 5 tablets weighing 449mg, 450mg, 448mg, 451mg and 453mg, respectively. Calculate 300 mg equivalent sample weight for assay. The product claim is 500 mg Vitamin C is present in each tablet. Question 2: Volume of the standard sodium edetate is 12.6 mL (calculated after the assay of Zinc Sulfate Syrup). Determine the % of potency of the drug considering the claimed dose is 11.5
- You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?why does threonine and serine test positive for biuret test?TRUE FALSE Keller-Killiani Test - Involves color reaction of the sugar 2- deoxyhexose Salkowski Test - The upper layer turns yellow with green fluorescence Liebermann-Burchard Test - Acetic anhydride reacts with the aliphatic hydrocarbon. Keller-Killiani Test - Color change results to reddish brown that turns into violet or purple Liebermann-Burchard Test - Color change is due to increasing conjugation of the double bond in adjacent fused ring Salkowski Test - The chloroform layer turns red. Liebermann-Burchard Test - Chloroform was added to react with sulfuric acid. Liebermann-Burchard Test - Must observe the color right away because it happens in a very short period of time. Salkowski Test - Involves the reagents chloroform, sulfuric acid and acetic anhydride Liebermann-Burchard Test - Reaction involved is esterification.
- AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?This is a molarity problem that I cannot figure out. If you could please help me understand the steps to solving it that would be greatly appreciated! Question: We need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Our liquid salt solution is supplied in a 50X concentration, and we need to dilute it to 1X for use. We also need to add 75% fetal bovine serum for a final concentration of 15%. How would we make up 0.80 liters of this culture media using water as our solvent?A thyroid peroxidase antibody test was performed on a 45 year old man. The dilution sequence was 25 microliters serum to 100 microliters of diluent in tube 1. Then 25 microliters from tube 1 was added to 25 microliters of diluent in tube 2. Then 25 microliters from tube 2 was added to 50 microliters of diluent in tube 3. What is the dilution factor of tube 1. What is the dilution factor in just tube 2. What is the dilution factor in just tube 3. What is the overall dilution factor in tube 3.
- 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?The accompanying figure is from an electrophoresis experiment using SDS–PAGE. The left lane has the following standards: bovine serum albumin (MW 66,000), ovalbumin (MW45,000), glyceraldehyde 3-phosphate dehydrogenase (MW 36,000),carbonic anhydrase (MW 24,000), and trypsinogen (MW 20,000).The right lane is an unknown. Calculate the MW of the unknown.You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL solution of bovine gamma globulin (BGG). From these solutions, you will need to make 100 μL of each of the following standards using 1x MBP purification buffer as the diluent for preparation of a standard curve: 1000 μg/mL, 750 μg/mL, 500 μg/mL, 250 μg/mL, 125 μg/mL, and 0 μg/mL.