Determine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml of 0.9% (w/v) saline (NaCl) b) 30 ml of 50% glycerol (v/v)   c) Electrophoresis requires TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. TAE is normally made as a 50X concentrated stock. Provide a recipe to make 40 ml of 50X TAE. The recipe for one liter of 50X TAE is as follows: 242g Tris base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTA

Determine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml of 0.9% (w/v) saline (NaCl) b) 30 ml of 50% glycerol (v/v)   c) Electrophoresis requires TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. TAE is normally made as a 50X concentrated stock. Provide a recipe to make 40 ml of 50X TAE. The recipe for one liter of 50X TAE is as follows: 242g Tris base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTA

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 7.2P

See similar textbooks

Similar questions

NEORAL oral solution contains 100 mg/mL of cyclosporine. If a pharmacist prepares 30 mL of an oral solution containing 10% w/v cyclosporine, how many milliliters of diluent should be used?
An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?
1) You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed. 2. How would you make up one liter of 1X TAE buffer using a 25X stock?
Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?
As a part of a clinical study, a pharmacist is asked to prepare a modification of a standard 22 g package of a 2% mupirocin ointment by adding the needed quantity of mupirocin powder to prepare a 3% w/w mupirocin ointment. How many mil- ligrams of mupirocin powder are required?
You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock solution is 5M. a)How would you make 300mL of a 1X solution? b)What is the concentration of NaCl in your 1X solution?
The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?
Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest experimental pH range used to seperate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: Ending pH:
Consider the following from the Ampicillin for Injection, USP package insert: For Intramuscular Use: Label Claim 250mg 500mg 1g 2g Amount of Diluent (mL) Approx. Volume (ml) 0.9 1.7 3.4 6.8 1 2 4 8 a) What is the amount of diluent needed to reconstitute a 1 g vial? b) What is the powder volume for a 1 g vial of ampicillin? Approx. Concentration (mg/mL) 250 250 250 250 Later in the package insert you see: For Direct Intravenous Use: Add 5 mL Sterile Water for Injection or Bacteriostatic Water for Injection to the 250 and 500 mg vials and administer slowly over a 3 to 5-minute period. Ampicillin for Injection, 1 g or 2 g, may also be given by direct intravenous administration. Dissolve in 7.4 or 14.8 mL Sterile Water for Injection or Bacteriostatic Water for Injection, respectively, and administer slowly over at least 10 to 15 minutes. c) What is the amount of diluent needed to reconstitute a 1 g vial of ampicillin for direct IV injection d) What is the total amount of volume that will…