Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Textbook Question
Chapter CHST3, Problem 3DQ
If you were acting as a defense lawyer in a murder case that used DNA profiling as evidence against the defendant, how would you explain to the jury the limitations that might alter their interpretation of the crime scene DNA profile?
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Should DNA phenotyping be used to identify or convict a suspect?
Why is DNA evidence more useful as exclusionary evidence than for positive identification of a suspect?
1) A forensic scientist is required to collect blood samples from the crime scene and
analyze it to help the investigators understand who participated to the murder.
i) As a first step, the scientist needs to extract DNA. Indicate why red blood cells are not a
good target for the analysis, and list at least two chemicals/compounds that are required to
extract the DNA, explaining why they are used.
ii) The sample has been collected in a small amount, so that (s)he decides to use PCR to
analyze it. Describe why PCR is better suited to address small samples with respect to
Southern blotting and how is selectivity addressed with this technique.
Chapter CHST3 Solutions
Essentials of Genetics (9th Edition) - Standalone book
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