Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter CHST3, Problem 2RQ
Summary Introduction
To review:
The reason behind using short tandem repeats (STRs) as a very common loci for
Introduction:
Microsatellites comprises a set of 2 to 13
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Why do you suppose that forensic DNA analysis relies principally onshort tandem repeats (repeat polymorphisms) rather than single-nucleotidepolymorphisms?
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- The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis ofshort tandem repeats, STRs, in the human genome. This approach has many distinct advantages.Please list and explain three of those advantages.arrow_forwardWhether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?arrow_forwardWhy is it important that the alcohol used in the DNA extraction is kept cold?arrow_forward
- What are STRs, and why are they useful for DNA profiling?arrow_forwardWhy are entire genomes not used for DNA profiling?arrow_forwardThe last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?arrow_forward
- What is the purpose of adding the dishwashing liquid to the DNA extraction?arrow_forwardwhat are the advantages and disadvantages of Gel electrophoresis in DNA analysisarrow_forwardThree pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be closest to the positive or negative electrode?arrow_forward
- What is the purpose of adding table salt (NaCl) to the DNA extraction buffer?arrow_forwardWhat is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method? ddATP ddCTP ddGTP ddTTParrow_forwardWhat is the application of DNA extraction in healthcare? Cite at least 3 specific examples.arrow_forward
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