Concepts of Genetics (11th Edition)
Concepts of Genetics (11th Edition)
11th Edition
ISBN: 9780321948915
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 9, Problem 1PDQ

HOW DO WE KNOW? In this chapter, we focused on extranuclear inheritance and how traits can be determined by genetic information contained in mitochondria and chloroplasts, and we discussed how expression of maternal genotypes can affect the phenotype of an organism. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?

  1. (a) How was it established that particular phenotypes are inherited as a result of genetic information present in the chloroplast rather than in the nucleus?
  2. (b) How did the discovery of three categories of petite mutations in yeast lead researchers to postulate extranuclear inheritance of colony size?
  3. (c) What observations support the endosymbiotic theory?
  4. (d) What key observations in crosses between dextrally and sinistrally coiled snails support the explanation that this phenotype is the result of maternal-effect inheritance?
  5. (e) What findings demonstrate a maternal effect as the basis of a mode of inheritance?
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As a volunteer in a genetics lab, Professor Uhura tasked you with testing the biparental mitochondria inheritance hypothesis in Drosophila for a metabolic phenotype that they are studying. Because you just got started in the lab, the professor tells you are not allowed to use any high-end high-tech resources to perform your experiments and to collect data. The only resources you have access to are the various stocks of Drosophila available in the incubators, the resources to feed the flies and to transfer the flies from vial to vial to perform crosses, and tools to measure the metabolic phenotype of interest.  Describe an experimental approach using crosses you could follow that could potentially provide you with data to support the biparental mtDNA inheritance hypothesis?
APOE gene has been found to be a major contributor to sporadic Alzheimer's disease (AD), by acting as an age-of-onset modifier for the common relatively late-onset forms of the disease. Among four alleles causing early onset of AD, the epsilon4 allele (APOE4) disrupts this function. If you generate transgenic monkeys in which the normal allele of APOE gene is knocked out, what phenotype will you expect for those knockout monkeys? A. The APE mRNA expression will be completely suppressed. B. They slow down the development of AD. C. They develop AD early. D. They don't show any AD symptom.

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Concepts of Genetics (11th Edition)

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY