Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 8.4, Problem 4CC
Summary Introduction

To draw: A graph depicting the rate of reaction for lysosomal enzyme whose internal pH is 4.5

Introduction: Lysosomes are the enzymes that are present in the cytoplasm and are used in breaking down of molecules such as carbohydrates. It also digests foreign agents during phagocytosis mechanism in many cellular organisms. It is also released during apoptotic reactions.

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The cell membrane is both fluid and asymmetrical. Discuss asymmetry of the plasma membrane, as well as the enzyme involved. Explain properties that contribute to the fluidity of the cell membrane?
How many copies of a protein need to be present in a cell in order for it to be visible as a band on an SDS gel? Assume that you can load 100 µg of cell extract onto a gel and that you can detect 10 ng in a single band by sil ver staining the gel. The concentration of protein in cells is about 200 mg/mL, and a typical mammalian cell has a volume of about 1000 μm³ and a typical bacterium a vol ume of about 1 µm³. Given these parameters, calculate the number of copies of a 120-kd protein that would need to be present in a mammalian cell and in a bacterium in order to give a detectable band on a gel. You might try an order-of-magnitude guess before you make the calcula tions.
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY