Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 8.4, Problem 1CR
Describe the events that occur on an agar plate containing a bacterial lawn when a single bacteriophage particle causes the formation of a bacteriophage plaque.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Which of the following is TRUE when one assay bacteriophage titers? You should:
a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate
b) directly add the phage dilution onto the surface of an agar plate
c) add tryptic soy broth to the phage dilution and incubate overnight
d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
In a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria.
why didn't the bacteriophage infect all 3 bacteria?
You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated
with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure
that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in
the media. What is the most likely explanation?
O You used the wrong bacteria or the wrong bacteriophage.
There is an issue with the phage batch.
O There is a problem with your bacterial culture of B. cereus.
O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.
Chapter 8 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 8.1 - How does a virus differ from a cell?Ch. 8.1 - Why does a virus need a host cell?Ch. 8.1 - Compared with cells, what is unusual about viral...Ch. 8.1 - Once inside a host prokaryotic cell, what are the...Ch. 8.2 - Distinguish between a capsid and a capsomere. What...Ch. 8.2 - What is the difference between a naked virus and...Ch. 8.2 - What kinds of enzymes can be found within the...Ch. 8.2 - Where does the envelope surrounding animal viruses...Ch. 8.3 - What is packaged into capsids during maturation?Ch. 8.3 - Explain the term burst size.
Ch. 8.3 - Prob. 3MQCh. 8.3 - Why does a one-step growth curve differ in shape...Ch. 8.4 - What is meant by a viral titer?Ch. 8.4 - What is a plaque-forming unit?Ch. 8.4 - What is meant by the term plating efficiency?Ch. 8.4 - Describe the events that occur on an agar plate...Ch. 8.5 - How does attachment contribute to virushost...Ch. 8.5 - Prob. 2MQCh. 8.5 - Prob. 3MQCh. 8.5 - What is required for a bacteriophage T4 virion to...Ch. 8.6 - Prob. 1MQCh. 8.6 - Give one example each of T4 early, middle, and...Ch. 8.6 - What is required to package the T4 genome into its...Ch. 8.6 - Bacteriophage T4 lacks its own RNA polymerase. How...Ch. 8.7 - What is a lysogen and what is a prophage?Ch. 8.7 - How does DNA replication in lambda differ from...Ch. 8.7 - What commits lambda to the lytic versus the...Ch. 8.7 - What enzyme is required to form a prophage, and...Ch. 8.8 - Prob. 1MQCh. 8.8 - What is the difference between a persistent and a...Ch. 8.8 - Prob. 3MQCh. 8.8 - Why can it be said that the retrovirus genome is...Ch. 8 - What causes the viral plaques that appear on a...Ch. 8 - The promoters on genes encoding early proteins in...Ch. 8 - Under some conditions, it is possible to obtain...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forwardStreptococcus pneumoniae is a Gram-positive bacterium that colonizes the mucosal surface of the upper respiratory tract in humans. The presence of this bacterium in the nose and throat is widespread in the population, and in most people, colonization with Strep. pneumoniae is asymptomatic. The figure attached shows a comparison of in vitro growth curves of the wild-type strain of Strep. pneumoniae, as well as a Strep. pneumoniae mutant strain with a defect in one bacterial gene. The graph on the right shows the growth curve following addition of lysozyme during the logarithmic phase of bacterial growth. Which statement could account for the data in these graphs? Strain B is wild-type Strep. pneumoniae, and strain A is a mutant that cannot modify its peptidoglycan to be lysozyme-resistant. Strain B is wild-type Strep. pneumoniae, and strain A is a mutant that that expresses increased levels of LPS. Strain A is wild-type Strep. pneumoniae, and strain B is a mutant that cannot modify its…arrow_forwardDescribe in detail the kinetics of phage infection followed by host lysis in bacteria. with examplesarrow_forward
- Transfection efficiency refers to a) total number of transfected cells per dish O b) total number of transfected cells per field of view total amount of plasmid DNA added per sample d) number of cells per 100 that have taken up plasmid DNAarrow_forwardA suspected pathogen is observed from the blood of a diseased mouse. An investigation of the other mice in the area indicates that it is not present in healthy mice. With Koch's postulates in mind, what is the next step to determine whether or not this suspected pathogen is the cause of disease in the mouse? a) Grow the suspected pathogen in a pure culture Ob) Re-isolate the suspected pathogen and show that it is the same as the original pathogen c) Inoculate a healthy mouse with the suspected pathogen O d) Determine whether the organism in the pure culture is the same one as in the original samplearrow_forwardList all the antibiotics in the above table that work through destroying the bacterial cell wall. (if there are none listed please state that)arrow_forward
- In conjugation of a Hfr cell with an F- cell: a) the container cell becomes F + b) The container cell remains F- c) The donor cell becomes F- d) The recipient cell becomes Hfrarrow_forward10 mL of a bacteriophage suspension is mixed with 5 mL of a bacterial culture and 25% of the phages adsorb. The bacteriophage suspension has a concentration of 4x1018 viruses per mL, and the bacterial culture had a concentration of 6x1014 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)? What fraction of the cells is uninfected? What fraction of the cells is single infected? What fraction of the cells is multiple infected?arrow_forwardWhich of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forward
- When T4 phage isolated from E. coli strain B infect E. coli strain K, what will be the EOP (efficient of plating)? Explain why. Fe Varrow_forwardYou take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?arrow_forwardThe volume of E. coli added to each warm agar pour/virus mixture was originally100µL. After pre-testing the experiment, the instructions were modified for you to add 300µL of E. coli. Make an educated guess as to why the volume was increased.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Infectious Diseases - How do we control them?; Author: Let's Learn Public Health;https://www.youtube.com/watch?v=2JWku3Kjpq0;License: Standard Youtube License