The volume of E. coli added to each warm agar pour/virus mixture was originally100µL.  After pre-testing the experiment, the instructions were modified for you to add 300µL of E. coli.  Make an educated guess as to why the volume was increased.

Q: A student completed a plate and liquid lysate followed by a plaque assay. The volume harvested for…

A: PFU is the amount of viral particles capable of forming plaques in a sample. PFU of a viral sample…

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: The following set of data are measurements of zones of inhibition (in mm) from a Kirby-Bauer test.…

A: The Kirby-Bauer test, also known as the disc diffusion test, is a standard method used to determine…

Q: The following three questions are related to this patient scenario: A 65-year-old male HIV patient…

A: The intravenous antibiotics are used to treat severe kinds of bacterial infections. A most common…

Q: In performing an agar overlay for E. coli bacteriophage isolation, formation of ____________…

A: The agar overlay is a common tool in the culture-based research of bacterial viruses…

Q: The world is facing a Corona Virus (COVID-19) Pandemic and as a potential scientist and a…

A: The Alcohol has both the anti-microbial as well as anti-viral properties and hence used as the…

Q: In Griffith's experiment, the S type bacteria are harmless, but can be transformed into the…

A: In Griffith's experiment, there was a mix of  heat-killed S-strain bacteria with live, harmless…

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: researcher wants to compare the pathogenicity of a mutant pathogen relative to wild type in an…

A: The term "pathogenicity" describes an organism's capacity to cause disease in its host. The overt…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: What were some of the conclusions of the Griffith experiment on streptococcus pneumonia?  Why was…

A: Griffith's experiment, published in 1928 by Frederick Griffith, was the first to propose that…

Q: You are treating swimming pools with ozone. You are using 0.02 ppm ozone to kill adenovirus, with an…

A: Adenoviruses are a group of common viruses that infect the lining of your eyes, airways and lungs,…

Q: Describe and explain the difference in results if the serial dilution step of the T4 bacteriophage…

A: The T4 bacteriophage is a type of virus that infects bacterial cells. It is a complex virus that has…

Q: You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a…

A: A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to…

Q: This agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken…

A: A colony-forming unit (CFU) is a unit that is used in microbiology to measure the viable number of…

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: You perform a plaque assay with your bacteria and serial dilutions of your bacteriophage,, and then…

A: Serial dilution is a process of making a series of dilution in order to determine the concentration…

Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg? Write…

A: Escherichia coli is a Gram-negative bacteria. It is present as normal intestinal flora but can also…

Q: Explain why only a virulent virus (like T4 phage), rather than a temperate virus, can be used in our…

A: If a virulent phage infects its host, it will produce more progeny as soon as phage DNA is injected…

Q: Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an…

A: The method of identifying an unknown bacterium is a precise one, requiring cautious perception and…

Q: What conclusion would you draw if the numbers of bacterial colonies in were the same on the control…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: If the concentration of phage in the original sample was markedly increased from the concentration…

A: 1. Dilution Series:  - Start with a high dilution, such as 10^-5 or 10^-6, of the original phage…

Q: You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase…

A: Enzymes can be defined as proteins that act as biological catalysts. They work by accelerating…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

A: For calculating the unknown concentration of the tube we will be using the formula as mentioned…

Q: A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL…

A: It is given that a culture of Staphylococcus is diluted as follows:1. The original 20mL of culture…

Q: In the crusade to create a vaccine for Poliomyelitis, Jonas Salk and Albert Sabin created two…

A: Solvents is the substance in which solute is dissolved thus forming a solution. Usually a solvent is…

Q: Sample A: Sample B: Sample C: Sample D: Sample E: Sample F: 22mm 18mm 35mm 30mm 28mm 28mm 21mm 17mm…

A: Introduction The zone of inhibition is the area around an antibiotic pot where bacteria cannot grow.…

Q: You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this…

A: Bacteria are prokaryotic organism and divides by binary fission that is responsible for doubling the…

Q: A COVID-19 test, which produce result in 10 minutes, has the following test characteristics. The…

A: This question involves analyzing the test characteristics and predictive values of a COVID-19 test…

Q: Suppose your professor handed you a test tube with 2.0 mL of M. foliorum infected with bacteriophage…

A:

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: In this talk, we will learn about a very important experiment called the Avery, McLeod, and McCarty…

Q: The organism tested on Blood Agar (plate shown furthest to the left) is: a. Micrococccus luteus b.…

A: INTRODUCTION Blood agar This is a solid culture medium consisting of blood, peptones and agar. In…

Q: One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts…

A: Real-time RT–PCR is a nuclear-derived test for detecting the presence of RNA or DNA of the pathogen…

Q: he following are the steps, listed in random order, for utilizing koch's postulates. what should be…

A: Koch's postulates were created in the nineteenth century as a set of basic instructions for…

Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…

A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: What is the genus and species of your bacteria? And What led you to confirm this result and why?…

A: My bacteria is Alcaligenes faecalis :  Genus of Alcaligenes faecalis :Alcaligenes Alcaligenes is a…

Q: You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal…

A: The evaluation of fecal microscopic organisms in water could be a critical public well-being measure…

Q: Consider a batch culture of sphere-shaped bacteria in a growth medium or broth, in which the mean…

A: Batch culturing of microorganisms is an important part of industrial applications of microbiology.…

Q: Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the…

A: Bacteriophages are viruses that infect bacteria, and the plaque assay is a commonly used technique…

Q: not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical…

A: Foot rash can occur as a result of an allergic reaction or infection. For example in athletes food…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A…

A: Bacteriophage : these are the viruses that attack on barterial cells and use them to produce more…

Q: late the initial titre of the sample viral stock if the followir Show clear sample calculations. bda…

A: Introduction  Infections that contaminate prokaryotic creatures, called bacteriophages or basically…

Q: A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This…

A: Concentration is the term used to define the amount of a substance present in the solution or the…

Reproductive system infection

The biological process through which parents produce new children is called reproduction. A reproductive system is a group of internal and external organs that collaborate to allow males and females to reproduce. Many experts believe that the reproductive system is one of the most important systems in the body due to its crucial function in the survival of the species. Among the body's basic systems, the reproductive tract is the one that differs the most between sexes, and it is also the only one that does not operate until puberty.