Concept explainers
(a)
Interpretation:
The number of moles of substrate that are hydrolyzed per second per milligram of enzyme when the substrate concentration is much greater than Km.
Concept introduction:
The region of the enzyme to which substrate molecules binds is known as the active site. The binding of substrate with the active site converts it into product molecules. Turnover number is the maximum number of substrate molecules that can be converted into products per catalytic site.
(b)
Interpretation:
The moles of active site in 1 mg of enzyme.
Concept introduction:
The region of the enzyme to which substrate molecules binds is known as the active site. The binding of substrate with the active site converts it into product molecules. Turnover number is the maximum number of substrate molecules that can be converted into products per catalytic site.
(c)
Interpretation:
The turnover number of the enzyme.
Concept introduction:
The region of the enzyme to which substrate molecules binds is known as the active site. The binding of substrate with the active site converts it into product molecules. Turnover number is the maximnumber of substrate molecules that can be converted into products per catalytic site.
Want to see the full answer?
Check out a sample textbook solutionChapter 8 Solutions
Biochemistry
- Crohn’s disease. Omega-3 fatty acids have been tested asa means to prevent relapse of Crohn’s disease. Two large,randomized, placebo-controlled studies have shown nosuch benefit from omega-3 fatty acids. Suppose you areasked to design an experiment to further study this claim.Imagine that you have collected data on Crohn’s relapsesin subjects who have used these omega-3 fatty acids and similar subjects who have not used them and that you canmeasure incidences of relapse for these subjects. Statethe null and alternative hypotheses you would use in yourstudy.arrow_forwardFo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase (lecture 22). The enzyme has 3 a-b and 12 ‘c’ subunits. The mitochondrion maintains Df=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. How much energy is available (from the proton electrochemical gradient) for ATP synthesis under these conditions (in kJ/mol)? What [ATP]/[ADP] ratio will be established at steady-state under these conditions? What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? Remember that full revolution of the crank (gamma subunit) produces 3 ATP.arrow_forwardHigh potential. What is the equilibrium ratio of phosphoenolpyruvate to pyruvate under standard conditions when [ATP ]/[ ADP ]=10? [ATP]/[ADP] = 10?arrow_forward
- Enteric bacteria .lactic acid, and propionic bacteria have distinctive metabolic traits that can be used to characterized and identify these organisms .Describe the metabolic characteristics of these organisms name a genus that belong to each group, initiated in what way these organisms can be differentiated.arrow_forwardNo free lunch. Explain why maintaining a high concentration of CO2 CO2 in the bundle-sheath cells of C4C4 plants is an example of active transport. How much ATP is required per CO2CO2 to maintain a high concentration of CO2CO2 in the bundle-sheath cells of C4C4 plants?arrow_forwardLess energetic electrons. Why are electrons carried by FADH2FADH2 not as energy rich as those carried by NADH? What is the consequence of this difference?arrow_forward
- Less energetic electrons. Why are electrons carried by FADH 2 not as energy rich as those carried by NADH? What is the consequence of this difference?arrow_forwardPernicious anemia. Purine biosynthesis is impaired by vitamin B12 B12 deficiency. Why? How might fatty acid and amino acid metabolism also be affected bya vitamin B12B12 deficiency?arrow_forwardJust Arrange. The enzyme serine hydroxymethyltransferase (SHMT) requires pyridoxal phosphate (PLP) as a cofactor. Arrange the steps of the likely mechanism for SHMT‑catalyzed serine degradation (producing glycine and N5,N10‑methylenetetrahydrofolate)arrow_forward
- Synthetic stoichiometries. What is the stoichiometry of the synthesis of... (a) ribose 5-phosphate from glucose 6- phosphate without the concomitant generation of NADPH? (b) NADPH from glucose 6-phosphate without the concomitant formation of pentose sugars?arrow_forwardBIOCHEMISTRY DRAWING. pls accept question only if 100% confident please and thank you. Enalapril is inactive until acted upon by an esterase. Draw the structure of the resulting bioactive derivative. Hintarrow_forwardFo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase . The enzyme has 3 alpha-beta and 12 ‘c’ subunits. The mitochondrion maintains change in membrane potential=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. . What [ATP]/[ADP] ratio will be established at steady-state under these conditions? What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? full revolution of the crank (gamma subunit) produces 3 ATP.arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning