Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 7, Problem 6TY
Summary Introduction

Introduction: Racker and Stoeckenicus conducted an experiment in which the orientation of bacteriorhodopsin was made such that H+ was pumped into a vesicle and each vesicle contains many molecules of bacteriorhodopsin.

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To study the mechanism of SERCA, you prepare membrane vesicles containing this protein oriented such that its ATP binding site is on the outer surface of the vesicle. To measure pump activity, you use an assay that detects the formation of inorganic phosphate in the medium. When you add calcium and ATP to the medium, you observe phosphate production for only a short period of time. Only after the addition of calcimycin , a molecule that makes membranes selectively permeable to calcium, do you observe sustained phosphate production. Explain.
Suppose you have a cell from a freshwater fish that is isotonic with its surrounding environment, with a K+ concentration inside the cell of 0.05M. You then immerse this cell in saltwater where the K+ concentration is 0.25M. What would you predict will happen to the cell? a)Water will move into the cell, and the cell will swell and burst b)Water will move out of the cell, and the cell will shrink c)Water will move in and out of the cell with no net change in concentration, and the cell will remain the same size d)The cell wall will prevent the cell from changing size
yeasts are able to produce high internal concentrations of glycerol to counteract the osmotic pressure of the surrounding media. suppose that a sample of yeast cells were placed in a 4% sodium chloride solution by weight.  The density of solution is at 25 C = 1.02 g/ml, Molecular weight of solute = 58.44 g/mol, i of glycerol = 1 and R=0.08205 L-atm/mol-K  What is the weight of solute in grams What is the moles of solute What is the volume of the solution in liters What is the molarity of the solution What is the value of the temperature to be used to solved for the osmotic pressure of the solution  What is the osmotic pressure of solution
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