Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 7, Problem 3A
A use-dilution test was used to evaluate two disinfectants against Salmonella choleraesuis. The results were as follows:
- a. Which disinfectant was the most effective?
- b. Which disinfectant should be used against Staphylococcus?
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Students have asked these similar questions
a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria
b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteria
The Kirby -Bauer antimicrobial sensitivity testing method relies on:
A. The inhibition of one antimicrobial by another
B. The diffusion of antimicrobials in paper discs
C. Serial dilutions of antimicrobials in test tubes
D. A gradient of an antimicrobial on a paper strip
Which one of the following methods of antimicrobial susceptibility testing combines some
of the properties of two other methods and involves adding a plastic strip to the agar?
Select one:
a. Agar macrodilution method
b. Gradient diffusion method
c. Broth microdilution method
d. Disk diffusion method
Chapter 7 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 7 - The thermal death time for a suspension of...Ch. 7 - If pasteurization does not achieve sterilization,...Ch. 7 - Thermal death point is not considered an accurate...Ch. 7 - Prob. 4RCh. 7 - DRAW IT A bacterial culture was in log phase in...Ch. 7 - How do autoclaving, hot air, and pasteurization...Ch. 7 - How do salts and sugars preserve foods? Why are...Ch. 7 - Prob. 8RCh. 7 - A large hospital washes burn patients in a...Ch. 7 - Prob. 10R
Ch. 7 - Which of the following does not kill endospores?...Ch. 7 - Which of the following is most effective for...Ch. 7 - Prob. 3MCQCh. 7 - Which of the following cannot be used to sterilize...Ch. 7 - Prob. 5MCQCh. 7 - The data were obtained from a use-dilution test...Ch. 7 - The data were obtained from a use-dilution test...Ch. 7 - Prob. 8MCQCh. 7 - A classmate is trying to determine how a...Ch. 7 - Which of the following is most likely to be...Ch. 7 - Prob. 1ACh. 7 - For each of the following bacteria, explain why it...Ch. 7 - A use-dilution test was used to evaluate two...Ch. 7 - To determine the lethal action of microwave...Ch. 7 - Entamoeba histolytica and Giardia lamblia were...Ch. 7 - Between March 9 and April 12five chronic...Ch. 7 - Prob. 3CAE
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- which kind of sample gives positive result for Molish test?arrow_forwardKoch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The symptoms of the test animal may vary from the symptoms seen in the O 2) patient. O 3) Inoculate a test animal with the isolated organism. 4) The organism must be isolated from the test animal in pure culture. 5) The organism isolated from the test animal must match the organism isolated from the patient.arrow_forwardA relatively large zone of inhibition surrounding an antimicrobial disk on a Kirby-Bauer test plate would most likely be interpreted as: a. sensitive reaction b. intermediate reaction c. resistant reactionarrow_forward
- What is the difference between a Hemin test and a Benzidine test?arrow_forwardGive the procedure of Sabin-Feldman dye test. Describe the positive and negative results.arrow_forwardWhat are the mechanism of action of hydrogen peroxide, rubbing alcohol, lab disinfectant and copper sulfate? Name a standard test for the effectiveness of a disinfectant. Name the chemical agent that is used as a standard. What are the standard test organisms?arrow_forward
- Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique 3. Ziehl-Neelsen stain 4. Lactophenol blue stain 5. Mehtamine silver technique 6. Shikata's orcein method 7. Mann's methyl blue-eosin stainarrow_forwardhow to find out the test is differential or selective in the microbiology lab test?arrow_forwardOther than the PYR test, what is another use of the PEP reagent?arrow_forward
- You saw no color change in both the first step and second step of the Nitrate Reduction test. What is the result of this experiment? Also, explain what happens when an excessive amount of zinc is added in the second step of this test.arrow_forwardAnswer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) Falsearrow_forwardA culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?arrow_forward
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USMLE-Rx Antimicrobial Therapy; Author: USMLE-Rx;https://www.youtube.com/watch?v=Pka1Ynlrqtw;License: Standard Youtube License