How would a high level of HCG present in a urine sample be indicated in this agglutination test?
To determine: How would a high level of hCG hormone available in a urine sample be represented in the agglutination test.
Introduction: Monoclonal antibodies refer to the antibodies that show specificity for a single epitope alone. In monoclonal antibody preparation, a vertebrate animal, typically a mouse is immunized with an antigen several times and is killed subsequently. The B lymphocytes are combined with cancerous multiple myeloma cells using a procedure described in 1975. These myeloma cells exhibit all the properties of plasma cells, but the immunoglobulins secretion. In a suitable medium, these cells can survive forever and they will multiply indefinitely. Thus, they continuously secrete identical, monoclonal antibodies in large quantities.
Explanation of Solution
The availability of huge amounts of pure monoclonal antibodies has let the development of highly sensitive clinical lab tests. A few pregnancy tests utilize a monoclonal antibody that is produced against the human chorionic gonadotrophin (hCG) hormone. The test utilizes hCG-coated latex particles and it is exposed to an anti-hCG antibody and a urine sample. If the urine sample includes high level of hCG hormone, it will hinder attachment of antibodies to the hCG-coated latex particles and prevent their clumping (agglutination), representing pregnancy depending on the availability of hCG.
Want to see more full solutions like this?
Chapter 50 Solutions
BIOLOGY
- What is the Biuret test actually testing? Be specific. Why is it important to include a water only sample for each test?arrow_forwardExplain the clinical significance and its normal and abnormal interpretation that could be associated with each reagent on the urine test strip .arrow_forwardThe test utilized for blood typing is?arrow_forward
- What are the clinical significance of red, black, and gray colored stool specimens?arrow_forwardYou are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).arrow_forwardSerum is diluted with an equal amount of diluent (such as tube #1, 1/2 and tube #2, 1/2). What is the concentration in tube #2 if the original concentration was 100 mg/dL?arrow_forward
- which of the following are necessary to run a valid elisa test?arrow_forwardWhat are the factors to consider when handling a stool sample? Also, in 6-7 sentences, discuss the techniques used in microscopic examination of stool.arrow_forwardIn the entrropluri tube system which test require the addition of reagents after the incubation time period?arrow_forward