Organic Chemistry Plus Masteringchemistry With Pearson Etext, Global Edition
Organic Chemistry Plus Masteringchemistry With Pearson Etext, Global Edition
9th Edition
ISBN: 9781292151229
Author: Wade, LeRoy G.
Publisher: PEARSON
Question
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Chapter 5, Problem 5.32SP

(a)

Interpretation Introduction

To determine: The specific rotation for the given sample solution on the basis of given information.

Interpretation: The specific rotation for the given sample solution is to be calculated on the basis of given information.

Concept introduction: Physical properties of a compound like boiling point, density affects the angular rotation of polarized light by a chiral compound. The concentration of the compound affects the rotation of polarized light in Polarimeter. The rotation is also dependent upon the length of the cell and optical activity of the compound.

(b)

Interpretation Introduction

To determine: The specific rotation for the given sample solution on the basis of given information.

Interpretation: The specific rotation for the given sample solution is to be calculated on the basis of given information.

Concept introduction: Physical properties of a compound like boiling point, density affects the angular rotation of polarized ligt by a chiral compound. The concentration of the compound affects the rotation of polarized light in Polarimeter. The rotation is also dependent upon the length of the cell and optical activity of the compound.

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A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
4. 1.00 g of sample is dissolved in 20.0 mL of ethanol. Then 5.00 mL of this solution is placed in a 20.0 cm polarimeter tube. The observed deviation angle of rotation is 1,25° counter clockwise. Calculate the specific rotation of the sample.
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
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