Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 4, Problem 7R
Summary Introduction
To review:
The cell wall components between gram-positive and gram-negative bacteria.
Introduction:
The cell wall of a bacteria is a slightly rigid structure that gives the shape of the cell. It is made up of an inner and an outer membrane which give protection to the cell from adverse conditions. The cell wall is present in most of the prokaryotes which serve as an attachment site of flagella, site to cause diseases and a site of antibiotic action.
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What will be the results of the following experiment with explanations:
A. Gram-positive bacteria and Gram-negative bacteria were placed into 0.2% NaCl solution.
B. Gram-negative bacterial isolate was placed into 0. 3% NaCl solution containing only Lysozyme enzyme.
C. Gram-positive bacteria and Gram-negative bacteria were placed into 0.09% NaCl solution containing only Penicillin?
Explain the difference between Gram positive and Gram negative cell walls.
Describe the procedure for performing a Gram stain.
What is the procedure?
How does each component work? (ie. What does the mordant do?)
How is the Gram stain used clinically to help identify the cause of an infection?
a. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below.
b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown results
Chapter 4 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 4 - DRAW IT Diagram each of the following flagellar...Ch. 4 - Endospore formation is called (a) _____. It is...Ch. 4 - DRAW IT Draw the bacterial shapes listed in (a),...Ch. 4 - Match the structures in column A to their...Ch. 4 - Why is an endospore called a resting structure? Of...Ch. 4 - Compare and contrast the following a. simple...Ch. 4 - Prob. 7RCh. 4 - Starch is readily metabolized by many cells, but a...Ch. 4 - Match the characteristics of eukaryotic cells in...Ch. 4 - NAME IT What group of microbes is characterized by...
Ch. 4 - Which of the following is not a distinguishing...Ch. 4 - Which statement best describes what happens when a...Ch. 4 - Which statement best describes what happens when a...Ch. 4 - Which statement best describes what happens when a...Ch. 4 - Which of the following statements best describes...Ch. 4 - Which of the following is false about fimbriae? a....Ch. 4 - Which of the following pairs is mismatched? a....Ch. 4 - Which of the following pairs is mismatched? a....Ch. 4 - You have isolated a motile, gram-positive cell...Ch. 4 - The antibiotic amphothericin B disrupts plasma...Ch. 4 - How can prokaryotic cells be smaller than...Ch. 4 - The smallest eukaryotic cell is the motile alga...Ch. 4 - Two types of prokaryotic cells have been...Ch. 4 - In 1985, a 0.5-mm cell was discovered in...Ch. 4 - When E. coli cells are exposed to a hypertonic...Ch. 4 - Clostridium botulinum is a strict anaerobe; that...Ch. 4 - A South San Francisco child enjoyed bath time at...Ch. 4 - Live cultures of Bacillus thuringiensis (Dipel)...
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