Campbell Biology: Concepts & Connections (9th Edition)
Campbell Biology: Concepts & Connections (9th Edition)
9th Edition
ISBN: 9780134296012
Author: Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan, Jane B. Reece
Publisher: PEARSON
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Chapter 4, Problem 2TYK
Summary Introduction

Introduction: Cell is the smallest fundamental unit of structure and function in all living organism. It is quite difficult to observe the ultra-structure of cell with naked eye. Inside the cell, cell-organelles are present; those are very minute to identify by electron microscope. So, high magnified electron microscope is needed for the observation of ultrastructure of cell and the cellular organelles.

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Which of the following cell components is not visible using a compound light microscope?   a. Nuclei b. Chloroplasts c. Mitochondria d. Vacuoles
In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.
Many microscopy techniques use cell characteristics, such as differences in absorption, refraction, or even light polarization. Fluorescence microscopy provides an image from a single molecular species, simply by looking at the fluorescence emission. How does fluorescence microscopy compare to other techniques? A.Image the location, diffusion or even transport of intracellular molecules.B.Accurately calculate the cytosol refractive index of a cellC.Count the number of cellsD.Accurately measure kernel size
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