Laboratory Manual For Human Anatomy & Physiology
4th Edition
ISBN: 9781260159363
Author: Martin, Terry R., Prentice-craver, Cynthia
Publisher: McGraw-Hill Publishing Co.
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Textbook Question
Chapter 4, Problem 2PL
The objective lenses of the compound light microscope are attached to the
- stage.
- base.
- body tube.
- rotating nosepiece.
Expert Solution & Answer
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Which of these is NOT a best practice when using, handling, or storing a microscope and microscope slides?
Do not touch the objective lenses.
Store the slides in bright sunlight to prevent mold growth.
Rotate the nosepiece to the lowest objective before storing the microscope.
Keep dust away from the scope by covering it during storage.
Put the steps for using a microscope in order.
While looking through the eyepiece, use the coarse adjustment knob to bring the object roughly into focus.
Lower the stage as far away from the objectives as possible.
Use the fine adjustment knob to bring the object into sharp focus.
Turn on the light.
Place the slide on the stage and center it.
Turn the nosepiece to the lowest power objective.
It is acceptable to use the course focus adjustment on a microscope when the scope is set
to high power.
True
False
Chapter 4 Solutions
Laboratory Manual For Human Anatomy & Physiology
Ch. 4 - The human eye cannot perceive objects less than...Ch. 4 - The objective lenses of the compound light...Ch. 4 - Prob. 3PLCh. 4 - Prob. 4PLCh. 4 - Basic lens cleaning is accomplished using water....Ch. 4 - When preparing a wet mount specimen for viewing,...Ch. 4 - The total magnification achieved using a 10...Ch. 4 - Prob. 8PLCh. 4 - Prob. 1.1ACh. 4 - Prob. 1.2A
Ch. 4 - Prob. 2.2ACh. 4 - If a circular object or specimen extends halfway...Ch. 4 - Prob. 3.2ACh. 4 - Prob. 3.3ACh. 4 - Why is it more difficult to measure the diameter...Ch. 4 - What change occurred in the intensity of the light...Ch. 4 - Prob. 3.6ACh. 4 - Prob. 3.7ACh. 4 - Prob. 3.8ACh. 4 - When you moved the ruler away from you, which way...Ch. 4 - Prob. F4.8ACh. 4 - Prob. 4.2A
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- briefly describe the functions of the following parts of the microscope: course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.arrow_forwardThe compound (bright-field) microscope is parfocal. It means that: Question 4 options: objective lenses are built into revolving nosepiece its lenses effectively decrease focal lenght can achieve maximum resolution lower than 0.2 μm image should remain in focus when objective lens is changedarrow_forwardMatch the part of the microscope with its use. Dragged and dropped options on the right-hand side will be automatically saved. For keyboard navigation... SHOW MORE Stage Nosepiece Vertical Stage Knobs Course and Fine Adjustment Knobs Iris Diaphram III ||| Allows for changing objective lenses Move the stage side to side Hold the slide in place Control light passing through the stage Move the stage up and downarrow_forward
- Which part of the microscope holds the slide or specimen? the body tube the condenser the fine adjustment knob the coarse adjustment knob the stagearrow_forwardUsing the scanning (4x) objective and the metric ruler, record the number of millimeters you see along with the letter “e.” Your value: 2 millimeters Convert the figure you attained to micrometers (1 millimeter = 1,000 micrometers). This is the diameter of the field of view for the low-power objective (LPD). The field of view is the circular field you see when you look through the oculars. The field of view changes at different magnifications. Your value (LPD): 2,000 micrometers -please help me with the problem in the picture.arrow_forwardTotal magnification of a microscope is determined by Multiple Choice dividing the objective magnification value by the ocular value. multiplying all objective magnification values together. adding all objective magnification values together. none of the above.arrow_forward
- The microscopic part that carries the objective lenses is the arm revolving nosepiece stage body tube base OOO OOarrow_forwardThe following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?arrow_forwardComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)arrow_forward
- Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30arrow_forwardPROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observationarrow_forwardList down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…arrow_forward
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