List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
1. Connect the microscope to the power supply. Turn “ON” the microscope.
2. Rotate the light intensity adjustment knob to adjust the brightness.
3. Place the slide with the specimen facing upwards on top of the mechanical stage.
a. Open the bow-shaped lever of the stage clip outward.
b. Slide the specimen from the front toward the rear.
c. Return the bow-shaped lever gently.
d. Center the specimen over the aperture on the stage.
4. Use the Low Power Objective.
a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.
b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.
c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.
d. When coarse focusing of the specimen is obtained, rotate the fine adjustment knob to adjust to precise focus.
e. Move the microscope slide until the image is in the center of the field of view. Then readjust the illuminator or diaphragm in order to attain maximum, comfortable level of light.
➢ Rotating the upper knob which is the Y-axis feed knob moves the specimen in the vertical direction. Rotating the lower knob which is the X-axis feed knob moves it in the horizontal direction.
➢ When using a binocular microscope, adjust the interpupillary distance and diopter, when necessary.
• Adjusting the interpupillary distance:
o While looking through the eyepieces, move both eyepieces until the left
and right fields of view coincide completely.
• Adjusting the diopter
o While looking through the right eyepiece with the right eye, rotate the
coarse and fine focus adjustment knobs to bring the specimen in focus.
o Then, while looking through the left eyepiece with the left eye, rotate the
diopter adjustment ring to focus on the specimen.
f. Once a clear image is attained under LPO, one should be able to change to a higher power objective lens with only minimal use of the focusing adjustment.
5. Use the High Power Objective.
a. Rotate the revolving nosepiece until the 40x objective lens is “clicked” into position.
b. Rotate the condenser focus knob to bring the condenser half-way up and open the iris diaphragm to attain the clear image.
c. While looking through the eyepiece, rotate the fine focus adjustment knob to bring the specimen into precise focus.
* Do not use the coarse adjustment knob.
d. When coarse focusing of the specimen is obtained, rotate the fine adjustment knob to adjust to precise focus.
6. Use the Oil Immersion Objective.
a. Before engaging the oil immersion objective in the light path, place a drop of immersion oil on the specimen at the area to be observed.
b. Rotate the revolving nosepiece in such a direction that the 10x and 40x objective lens never come in contact with the oil on the slide, until the 100x objective lens is “clicked” into position. The oil immersion objective lens should dip into the oil slightly.
c. Rotate the condenser focus knob to bring the condenser up and open the iris diaphragm fully to attain the clearest image.
d. While looking through the eyepiece, rotate the fine focus adjustment knob to bring the specimen into precise focus.
* Do not use the coarse adjustment knob.
e. After use, remove the oil from the objective front lens by wiping with lens paper or gauze slightly moistened with 70% alcohol.
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