Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
4th Edition
ISBN: 9781260159363
Author: Martin, Terry R., Prentice-craver, Cynthia
Publisher: McGraw-Hill Publishing Co.
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Chapter 4, Problem 3.3A
Summary Introduction

To analyze:

The diameter of the scanning power field of view compare with that of the low power field.

Introduction:

Magnification is the ability of a lens to magnify the object. It is the degree of visual enlargement that is expressed as a ratio of the size of the image to that of an object. It is measured by the multiples of 2x , 4x and 10x . The complete magnification of a microscope depends on the eyepieces and the objective lens.

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What is the total magnification of 4x objective lens and diameter of field of view in micrometers?
Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?
Indicate how the resolving power is determined and how resolution aff ects image visibility.
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