Laboratory Manual For Human Anatomy & Physiology
4th Edition
ISBN: 9781260159363
Author: Martin, Terry R., Prentice-craver, Cynthia
Publisher: McGraw-Hill Publishing Co.
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Textbook Question
Chapter 4, Problem 2.4A
If a circular object or specimen extends halfway across the scanning field, what is its diameter in millimeters?
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If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image?
10x
43x
1000x
400x
Define each of the following terms:
A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample?
B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece?
C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
Chapter 4 Solutions
Laboratory Manual For Human Anatomy & Physiology
Ch. 4 - The human eye cannot perceive objects less than...Ch. 4 - The objective lenses of the compound light...Ch. 4 - Prob. 3PLCh. 4 - Prob. 4PLCh. 4 - Basic lens cleaning is accomplished using water....Ch. 4 - When preparing a wet mount specimen for viewing,...Ch. 4 - The total magnification achieved using a 10...Ch. 4 - Prob. 8PLCh. 4 - Prob. 1.1ACh. 4 - Prob. 1.2A
Ch. 4 - Prob. 2.2ACh. 4 - If a circular object or specimen extends halfway...Ch. 4 - Prob. 3.2ACh. 4 - Prob. 3.3ACh. 4 - Why is it more difficult to measure the diameter...Ch. 4 - What change occurred in the intensity of the light...Ch. 4 - Prob. 3.6ACh. 4 - Prob. 3.7ACh. 4 - Prob. 3.8ACh. 4 - When you moved the ruler away from you, which way...Ch. 4 - Prob. F4.8ACh. 4 - Prob. 4.2A
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- If a circular object or specimen extends halfway across the scanning field, what isits diameter in millimeters? ___________arrow_forwardThese cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmarrow_forwardWhat is the objective lens used to locate the specimen and first focus? What is the difference in using a coarse adjustment knob and a fine adjustment knob?arrow_forward
- e) What is the total magnification of a specimen when viewed with the following objective: 400 Scanning High Power 400 Low power Oil Immersionarrow_forwardWhat is the specific objective lens that is used to locate the specimen and first focus? Explain. What is the difference between using a coarse adjustment knob and a fine adjustment knob?arrow_forwardWhat is the diameter of field of view for the high-power objective in millimeters and micrometers?arrow_forward
- If you are using a microscope and a specimen takes up 1/4 of the field of vview at 1000X, what is the diameter?arrow_forwardIf the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?arrow_forwardHow do you identify which objective lens are you using to view your sample?arrow_forward
- If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUarrow_forwardHow will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityarrow_forwardCalculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length umarrow_forward
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