What is the specific objective lens that is used to locate the specimen and first focus? Explain. What is the difference between using a coarse adjustment knob and a fine adjustment knob?
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- What is the specific objective lens that is used to locate the specimen and first focus? Explain.
- What is the difference between using a coarse adjustment knob and a fine adjustment knob?
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- What is the objective lens used to locate the specimen and first focus? What is the difference in using a coarse adjustment knob and a fine adjustment knob?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece, Revolving Nosepiece, Objective Lens, Stage and Condenser? Explain each
- What information can be obtained from the slides prepared by the wet mount technique and the hanging mount technique? Give at least three (3) information. What are the limitations of each method (Provide two limitations)If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying power of the objective being used?When using the oil immersion lens, what four procedures can be implemented to achieve the maximum resolution?
- What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece, Revolving Nosepiece, Objective Lens, Stage, Illuminator, Base and Condenser? Explain eachWhat is the magnification power of the objective lenses? How does the eyepiece compare to the objective lens? What is a magnification power? Differentiate between the fine and the coarse adjustment knobs.To obtain a 400X magnification image you may choose a 40X objective lens with a 10X projector lens, or a 20X objective lens with a 20X projector lens. What are the differences in their image quality?
- Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30How does the numerical aperture of an objective lens affect resolution? Question options: Numerical aperture is the optical plane visible for each objective lens Numerical aperture decreases the amount of excess light on a specimen increasing the resolution and clarity Numerical aperture is the loss of light due to changes in density between the specimen and the objective lens Numerical aperture is a measure of the amount of light an objective lens can accept producing higher resolution and allowing smaller structures to be visualized with greater clarityUsing the Field of View (FoV) formula: (Low Magnification/High Magnification)= (High Field of View/Low Field of View) determine the FoV in microns for all other objective lenses (4x,10x,40,100x) given that the diameter of the 4X scanner FoV is 3.2 mm Please, explain with the formula how it was used and how values were added
