Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Textbook Question
Chapter 4, Problem 1.3SC
Why would radio waves and microwaves be unsuitable for examining microbes?
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If you were trying to visualize living organisms without staining, which type of
microscopy would be best to utilize?
O 1) brightfield
O 2) TEM
3) fluorescent
O 4) confocal
O 5) phase contrast
What are the consequences if aseptic techniques are not strictly followed in performing microbiology experiments?
Question 1: Describe what you see through the microscope as compared to how the word "roe" looked when you placed the slide on the stage.
a) Describe what happened to the image as the total magnification was increased. In other words, how much of the specimen (the word roe) is visible at each level of magnification, 4x, 10x, and 40x?
Question 2: Given the images you have seen, please consider how it would be to need to find the red and white blood cells on this slide. List at least 3 observations for the blood slide. When discussing a specific image, make sure to note the Total Magnificiation (TM).
a) List another three observations below based on the bacterial slide images. Note the Total Magnification used when comparing blood and bacterial slides.
Chapter 4 Solutions
Microbiology: Principles and Explorations
Ch. 4 - Prob. 1.1SCCh. 4 - Prob. 1.2SCCh. 4 - Why would radio waves and microwaves be unsuitable...Ch. 4 - Would standard immersion oil placed on a plastic...Ch. 4 - Prob. 2.1SCCh. 4 - How can you distinguish between TEM and SEM...Ch. 4 - Prob. 2.3SCCh. 4 - Prob. 2.4SCCh. 4 - What can be observed in a wet-mount or...Ch. 4 - What is the difference between simple stains and...
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- What is the reason for using blind tube in spectrophotometric and calorimetric studies?arrow_forwardWhat are the advantanges and disadvantages of using stained preparations for observing microbial cell morphology?arrow_forwardWhy is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?arrow_forward
- Why is it important to use standards or to develop a standard curve in spectrophotometry?arrow_forwardWhat is the difference between culture media and cultural environment? Are environmental conditions related to specific microbes? If so, which conditions for which type of microbes? If you were a field researcher collecting microbes in the wild, what kind of data would you take to make sure you know the exact environment the samples require in order to culture them in a lab?arrow_forwardDoes it reduce, deaden, or enhance human creativity using Titan 80-300 Cubed Microscope?arrow_forward
- What are the significance of UV/Vis spectrophotometer in the experiment complexation.arrow_forwardwhat is the purpose of using oil immersion with the 100x objective lens? a)To prevent refraction of light b)To prevent scratching the slide c)To allow microbes to resuspend in the fluid d)Oil is not used with the 100x, but rather 40x e)To prevent microbes from dryingarrow_forwarddiscuss the limitations in using the light microscope to study microbial communities.arrow_forward
- What are advantages and disadvantages of solid phase bioremediation technique?arrow_forwardWhat is a micrometer (μm )? Is it really possible to see microbes with this dimension using Leeuwenhoek’s “primitive” microscopes?arrow_forwardWhat are the advantages and disadvantages of culture-dependent and culture-independent methods in microbiology, respectively.arrow_forward
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