Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 4, Problem 3.1SC
What can be observed in a wet-mount or hanging-drop preparation that cannot be oserved in heat-fixed slides?
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Chapter 4 Solutions
Microbiology: Principles and Explorations
Ch. 4 - Prob. 1.1SCCh. 4 - Prob. 1.2SCCh. 4 - Why would radio waves and microwaves be unsuitable...Ch. 4 - Would standard immersion oil placed on a plastic...Ch. 4 - Prob. 2.1SCCh. 4 - How can you distinguish between TEM and SEM...Ch. 4 - Prob. 2.3SCCh. 4 - Prob. 2.4SCCh. 4 - What can be observed in a wet-mount or...Ch. 4 - What is the difference between simple stains and...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.arrow_forwardExplain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.arrow_forwardWhat is the advantage of phase-contrast microscopyover bright-field microscopy?arrow_forward
- Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.arrow_forwardThe above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective of a compound microscope. What is the size of the circled cheek cell in millimeters AND nanometers?arrow_forwardWhy is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?arrow_forward
- Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?arrow_forwardWhat are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?arrow_forwardWould phase microscopy be of any particular value when examining a stained specimen? Explain.arrow_forward
- The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?arrow_forwardWhat is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?arrow_forwardHow many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?arrow_forward
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