Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 4, Problem 2.4SC
Summary Introduction
To rank: The microscopes fluorescent, bright-field, and TEM based on the wavelength of the illuminating beam used by them, starting with the longest wavelength.
Introduction: The microscope is an instrument that helps to view objects that are very minute to be viewed by the unaided human eye. The different microscope types are bright-field, dark-field, fluorescence, electron (TEM and SEM), confocal, digital, scanning tunneling, and Nomarski microscope.
Summary Introduction
To determine: The effect of wavelength on the resolving powers of the given microscopes.
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Chapter 4 Solutions
Microbiology: Principles and Explorations
Ch. 4 - Prob. 1.1SCCh. 4 - Prob. 1.2SCCh. 4 - Why would radio waves and microwaves be unsuitable...Ch. 4 - Would standard immersion oil placed on a plastic...Ch. 4 - Prob. 2.1SCCh. 4 - How can you distinguish between TEM and SEM...Ch. 4 - Prob. 2.3SCCh. 4 - Prob. 2.4SCCh. 4 - What can be observed in a wet-mount or...Ch. 4 - What is the difference between simple stains and...
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- What are the different magnifications of an ordinary light microscope?arrow_forwardThe magnification possible with any type of microscope is an important property, but its resolution, the ability to distinguish between two very closely apposed objects, is even more critical. Describe why the resolving power of a microscope is more important for seeing finer details than its magnification.arrow_forwardHow does a phase-contrast microscope increase contrast?arrow_forward
- What’s the aim of using a microscope for examining? What can the kohler illumination technique tell us? What are the sources of error that can impact the data ?arrow_forwardWhat is the advantage of phase-contrast microscopyover bright-field microscopy?arrow_forwardLabel the parts of the microscope on the diagram below: arm, stage, base, ocular, revolving nosepiece, objective (4x, 10x, 40x), course focus, fine focus, condenser & iris diaphragm (these are marked by the same line)arrow_forward
- Alternatives used on a light microscope might improve vision by using a different light source. Name two types of light sources.arrow_forwardWhat description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.arrow_forwardWhat’s the total magnification for 5x and 44x in a microscopearrow_forward
- How many types of objective lenses a light microscope has?arrow_forwardDescribe how the images obtained using a confocal microscope would appear different to those obtained using a widefield microscope.arrow_forwardList the optical parts of the microscope. How does it achieve magnification? Resolution?arrow_forward
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