Campbell Essential Biology with Physiology (6th Edition)
6th Edition
ISBN: 9780134711751
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 3, Problem 19PS
Summary Introduction
To describe: Tests that can help in determining which versions of lactose having an altered amino acid residue have a significantly altered three-dimensional structure.
Introduction:
Lactase in a digestive enzyme found in the human body. It hydrolyzes a disaccharide called lactose. Lactose is a disaccharide found richly in milk. Its hydrolysis yields two monosaccharides called glucose and galactose. Basically, the proteins are three-dimensional structures that are denoted by the order of the amino acids in its chain. The final conformational folded structure is driven by any polypeptide chain.
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Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids.
___1___--_2_-
3
--
--____6_--_7__
a. The amino acid in the C-terminal is optically inactive.
b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein but produces a yellow soln with basic hydrolysate. It also gives a negative
result with both Hopkin's Cole and Millon's test using basic hydrolysate.
c. After treatment of this peptide with aminopeptidase (specificity: peptide bond on the N-terminus), it yields serine and a hexapeptide
d. The amino acid besides the N-terminal amino acid. will yield ornithine and urea after alkaline hydrolysis.
e. The third amino acid gives a positive result for Hopkins-Cole Test.
f. Chymotrypsin^ cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue.
g. The amino acid at the carboxy-end of the third amino acid will yield a…
Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids.
a. The amino acid in the C-terminal is optically inactive.
b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein while producing a yellow soln with
Xanthoproteic reagent and yielding a negative result with Hopkin's Cole test using basic hydrolysate.
c. Yields serine and a hexapeptide after treatment of aminopeptidase (specificity: peptide bond on the N-terminus).
d. The amino acid beside the N-terminal a.a. will yield ornithine and urea after alkaline hydrolysis.
e. The third amino acid gives a positive result for Hopkins-Cole Test.
f. Chymotrypsin cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue.
g. The amino acid at the carboxy-end of the third a.a. will yield a black precipitate for Pb(Ac)2.
What is protein denaturation? Give five agents that can denature proteins
Is it possible for a denatured protein to revert into its native/original form? Please explain
Chapter 3 Solutions
Campbell Essential Biology with Physiology (6th Edition)
Ch. 3 - Prob. 1SQCh. 3 - Monomers are joined together to form larger...Ch. 3 - Prob. 3SQCh. 3 - Prob. 4SQCh. 3 - When two molecules of glucose (C6H12O6) are joined...Ch. 3 - One molecule of dietary fat is made by joining...Ch. 3 - Prob. 7SQCh. 3 - Prob. 8SQCh. 3 - Prob. 9SQCh. 3 - Most proteins can easily dissolve in water....
Ch. 3 - Prob. 11SQCh. 3 - Prob. 12SQCh. 3 - Name three similarities between DNA and RNA. Name...Ch. 3 - Prob. 14SQCh. 3 - Prob. 15IMTCh. 3 - Prob. 16IMTCh. 3 - Prob. 17IMTCh. 3 - A food manufacturer is advertising a new cake mix...Ch. 3 - Prob. 19PSCh. 3 - Prob. 20PSCh. 3 - Prob. 21BSCh. 3 - Prob. 22BSCh. 3 - Prob. 23BS
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