Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 28, Problem 2P

The Enzymatic Activities of DNA Polymerase I (a) What are the respective roles of the 5 -exonudease and 3 -exonuclease activities of DNA polymerase I? (b) What might be a feature of an E. coli strain that lacked DNA polymerase I 3 -exonuclease activity?

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2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA Polymerase III, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. coli strain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-functional) - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells without DNA Pol III differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.
DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its absence. (a) What is the mechanism of this reaction, and why is it dependent on AMP? (b) How might one determine that supercoiled DNA had in fact been relaxed?
DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its absence. (a) What is the mechanism of this reaction, and why does it depend on AMP? (b) How could you determine that supercoiled DNA had in fact been relaxed?
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Biochemistry
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