Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 26, Problem 11E
Summary Introduction

To design: An oligonucleotide-based affinity chromatography system to purify mRNA from cell lysate.

Introduction: Affinity chromatography is a technique used for high selectivity separation of biomolecules through their specific interactions. It is based on the unique chemical structure of given molecule. During this procedure, the interacting partner of the biomolecule is immobilized on a chromatographic resin. This ligand that is fixed to the stationary phase binds the desired biomolecule present in the mobile phase. The material can be eluted from the column by varying the composition of the mobile phase.

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