Chapter 25.3, Problem 3TY

Interpretation Introduction

Interpretation:

The length of the column that has to be packed with $\text{2}\text{μm}$ particles to have the same plate number has to be identified and the time taken for separation has to be calculated.

Concept Introduction:

Plate number is measure of peak dispersion on a HPLC column.  This reflects the column performance.  We can also find the efficiency of the column with this.  Plate number can be calculated using the formula given below,

$N\approx \frac{\text{3000 }•\text{ L(cm)}}{{\text{d}}_{\text{p}}(\mu \text{m)}}$

Where,

$\text{L}$ is the length of column

${\text{d}}_{\text{p}}$ is the size of the particles that is packed.