Interpretation:
The starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction has to be given.
Concept Introduction:
Reversed-phase chromatography:
One of the most common mode of HPLC is the reversed-phase chromatography. In reversed-phase chromatography, the mobile phase is more polar and the stationery phase is nonpolar or weakly polar. The solute is more rapidly eluted from the column in reversed-phase chromatography as the mobile phase is polar. Less polar mobile phase (solvent) has a high mobile phase strength.
This method is adequate to separate mixtures of low molecular mass.
To give the starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction
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