Chapter 25, Problem 25.4P

(a)

Interpretation Introduction

Interpretation:

The reason for requirement of high pressure in HPLC has to be given.

Concept Introduction:

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

(b)

Interpretation Introduction

Interpretation:

Smaller particles gives higher plate number for the given fixed column length has to be explained.

Concept Introduction:

Plate number is measure of peak dispersion on a HPLC column.  This reflects the column performance.  We can also find the efficiency of the column with this.  Plate number can be calculated using the formula given below,

$N\approx \frac{\text{3000 }•\text{ L(cm)}}{{\text{d}}_{\text{p}}(\mu \text{m)}}$

Where,

$\text{L}$ is the length of column

${\text{d}}_{\text{p}}$ is the size of the particles that is packed.

(c)

Interpretation Introduction

Interpretation:

Bonded phase in liquid chromatography has to be explained.

Concept Introduction:

Liquid chromatography is one of the important technique that is used in analytical chemistry.  By using this technique, the components present in the given sample can be separated.  The components are separated based on their interaction with stationery and mobile phase.