Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 22.5, Problem 1COMQ
Summary Introduction
Introduction:
Shotgun sequencing is a method that is used for deoxyribonucleic acid (DNA) sequencing. Fred Sanger and his colleagues used shotgun sequencing for the first time to sequence the genome of microscopic organisms like, viruses and bacteria with small genetic material. The first organism that was sequenced using shotgun was Hemophilus influenzae. Craig Venter also used this method to sequence the human genome.
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Shotgun sequencing is a method of DNA sequencing in whicha. the DNA fragments to be sequenced are randomly generatedfrom larger DNA fragments.b. the sequencing reactions are carried out in rapidsuccession.c. the samples to be sequenced are rapidly generatedby PCR.d. all of the above occur.
When we discuss PCR and other similar techniques, the term Tm is often used. This refers to
a.The temperature where the DNA molecules denature.
b.The temperature of the first step in a PCR cycle.
c.The temperature where half of the DNA molecules are denatured.
d.Tm is the same as the annealing temperature.
e.The Temperature at which all of the DNA molecules are dentatured
Which of the following is true?
a.
In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore.
b.
Next generation sequencing is slow but accurate.
c.
Both of the above are true.
Chapter 22 Solutions
Genetics: Analysis and Principles
Ch. 22.1 - Prob. 1COMQCh. 22.2 - Prob. 1COMQCh. 22.3 - A molecular marker is a _____ found at a specific...Ch. 22.3 - 2. Which of the following is an example of a...Ch. 22.3 - To map the distance between molecular markers via...Ch. 22.4 - 1. What is a contig?
a. A fragment of DNA that...Ch. 22.4 - A vector that can carry a large fragment of...Ch. 22.4 - 3. Chromosomal walking is a method of _____ in...Ch. 22.5 - Prob. 1COMQCh. 22.5 - Prob. 2COMQ
Ch. 22.5 - 3. A prokaryotic genome is about 4 million bp in...Ch. 22.6 - Metagenomics is aimed at a. determining the...Ch. 22 - 1. A person with a rare genetic disease has a...Ch. 22 - For each of the following, decide if it could be...Ch. 22 - Which of the following statements about molecular...Ch. 22 - 1. Is each of the following a method used in...Ch. 22 - Prob. 2EQCh. 22 - Prob. 3EQCh. 22 - The cells from a persons malignant tumor were...Ch. 22 - 5. Figure 23.2 describes the technique of FISH....Ch. 22 - Explain how DNA probes with different fluorescence...Ch. 22 - 7. A researcher is interested in a gene found on...Ch. 22 - Prob. 8EQCh. 22 - Prob. 9EQCh. 22 - Prob. 10EQCh. 22 - Prob. 11EQCh. 22 - Prob. 12EQCh. 22 - In the Human Genome Project, researchers have...Ch. 22 - 14. Take a look at question 3 in More Genetic...Ch. 22 - 15. Place the following stages of a physical...Ch. 22 - 16. What is an STS? How are STSs generated...Ch. 22 - 17. Four cosmid clones, which we will call cosmids...Ch. 22 - A human gene, which we will call geneX, is located...Ch. 22 - 19. Describe how you would clone a gene by...Ch. 22 - 20. A bacterium has a genome size of 4.4 Mb. If a...Ch. 22 - 21. Discuss the advantages of next-generation...Ch. 22 - Prob. 23EQCh. 22 - Prob. 24EQCh. 22 - Prob. 15EQCh. 22 - What is a molecular marker? Give two examples....Ch. 22 - Which goals of the Human Genome Project do you...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forwardChoose the basic chemical materials that are required to perform PCR. A. Two oligonucleotide primers. B. DNA fragment to be amplified. CA heat-resistant DNA polymerase. D. All of the abovearrow_forwardWhat would be the effect of performing a RT-PCR with the following ingredients: an mRNA template, appropriate primers, dNTPs, heat-stable reverse transcriptase and human DNA polymerase? Select one: a. The PCR would occur, but with a high mutation rate b. The PCR reaction will not commence c. Non-specific PCR of random templates will occur d. The RNA template would be converted to DNA, but the DNA segment would not be amplified PCR would proceed normally е.arrow_forward
- Transcriptomes, that is, all the transcripts in a cell, are analyzed using: a. a type of PCR b. fluorescently labeled nucleotides c. microarrays d. all the abovearrow_forwardWhat is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the abovearrow_forwardMatch the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forward
- The CRISPR method is used in a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the polymerase chain reaction.arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardWhat are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forward
- Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardPCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller fragments. O b. Determine if a particular piece of DNA contains a certain gene. Oc. Amplify a specific segment of DNA. d. Separate DNA fragments based on size. e. Isolate specific pieces of DNA. ess Jump to...arrow_forwardWhich of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forward
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