Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 22.2, Problem 1COMQ
Summary Introduction
Introduction:
Fluorescence, in the situ hybridization (FISH) technique, is used to map the location of certain genes. In this technique, the chromosomal deoxyribonucleic acid (DNA) is first denatured, and then, a probe is added to it. FISH is used for the detection of various diseases such as, phenylketonuria and various cancerous diseases which, are caused by mutation of genes.
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Which of the following is NOT used in an in situ hybridization experiment for visualizing DNA? (CSLO 1, CSLO 3, CSLO 4, CSLO 7)
O A. chromosome spread
O B. probe
O C. denaturation solution
D. antibody
E. microscope
How can you detect 1 small, very specific, piece of DNA (eg: a gene), among THOUSANDS of other pieces or sequences of DNA from nuclear chromosomes?
a. DNA hybridization
b. RNA hybridization
c. Use of a radioactive or fluorescent probe
d. Colony blotting and hybridization
e. Synthetic primers and PCR
f. Any of the above methods
The in situ hybridization method is based on
Select one:
a. labeling specific neuronal components with antibodies
b. injecting a fluorescent dye into a neuron
c. nucleic acid chains complementary to mRNAs that encode specific genes
d. using nucleic acid probes to detect specific proteins
e. formation of an insoluble colored product within cell bodiesc
Chapter 22 Solutions
Genetics: Analysis and Principles
Ch. 22.1 - Prob. 1COMQCh. 22.2 - Prob. 1COMQCh. 22.3 - A molecular marker is a _____ found at a specific...Ch. 22.3 - 2. Which of the following is an example of a...Ch. 22.3 - To map the distance between molecular markers via...Ch. 22.4 - 1. What is a contig?
a. A fragment of DNA that...Ch. 22.4 - A vector that can carry a large fragment of...Ch. 22.4 - 3. Chromosomal walking is a method of _____ in...Ch. 22.5 - Prob. 1COMQCh. 22.5 - Prob. 2COMQ
Ch. 22.5 - 3. A prokaryotic genome is about 4 million bp in...Ch. 22.6 - Metagenomics is aimed at a. determining the...Ch. 22 - 1. A person with a rare genetic disease has a...Ch. 22 - For each of the following, decide if it could be...Ch. 22 - Which of the following statements about molecular...Ch. 22 - 1. Is each of the following a method used in...Ch. 22 - Prob. 2EQCh. 22 - Prob. 3EQCh. 22 - The cells from a persons malignant tumor were...Ch. 22 - 5. Figure 23.2 describes the technique of FISH....Ch. 22 - Explain how DNA probes with different fluorescence...Ch. 22 - 7. A researcher is interested in a gene found on...Ch. 22 - Prob. 8EQCh. 22 - Prob. 9EQCh. 22 - Prob. 10EQCh. 22 - Prob. 11EQCh. 22 - Prob. 12EQCh. 22 - In the Human Genome Project, researchers have...Ch. 22 - 14. Take a look at question 3 in More Genetic...Ch. 22 - 15. Place the following stages of a physical...Ch. 22 - 16. What is an STS? How are STSs generated...Ch. 22 - 17. Four cosmid clones, which we will call cosmids...Ch. 22 - A human gene, which we will call geneX, is located...Ch. 22 - 19. Describe how you would clone a gene by...Ch. 22 - 20. A bacterium has a genome size of 4.4 Mb. If a...Ch. 22 - 21. Discuss the advantages of next-generation...Ch. 22 - Prob. 23EQCh. 22 - Prob. 24EQCh. 22 - Prob. 15EQCh. 22 - What is a molecular marker? Give two examples....Ch. 22 - Which goals of the Human Genome Project do you...
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- Linkage analysis ________. A. is used to create a physical map B. Involves breaking and rejoining of DNA artificially (incorrect) C. Requires radiation hybrid mapping D. Is based on natural recombination process thank you in advance for your help!arrow_forwardIn step 2 (above) in the identification of the colony that contains a targeted gene, ____. a. bacterial cells are cloned b. cells with the targeted gene are identified c. bacterial colonies are transferred d. DNA is lysed e. bacterial cells are rupturedarrow_forwardWhich of the following complex compound is used in the treatment of cancer?A. Cis platin B. Transplatin C. Potassium ferrocyanide D. Sodium cobal nitritearrow_forward
- Lampbrush and polytene chromosomes are characteristic because of the fact that A. nucleosomes are 3-dimensionally distinct from "normal" chromosomes B. microscopy shows they have regions with loosely bound DNA near tightly bound regions C. multiple centromeres were discovered on their linear sequence by in situ hybridization D. DNAse is unable to cleave the linkage between strandarrow_forwardUsing one of the options below, finish the following statement so that it is correct. Plasmids are _________ a. extrachromosomal, circular molecules of RNA b. extrachromosomal, linear molecules of DNA c. None of these options are correct d. extrachromosomal, circular molecules of DNA e. extrachromosomal, linear molecules of RNAarrow_forwardChoose the one answer that fits best. Which of the following statements regarding bacteria is NOT correct? a. During conjugation, two bacteria exchange genetic information directly via pili b. Bacterial populations have a higher mutation rate because their DNA Polymerases do not have proofreading capability c. Bacterial growth by binary fission is exponential and each division usually takes only a couple of hour or less d. Plasmids carry just a few genes and are replicated independently from the bacterial chromosome e. Cocci that are arranged in clusters/bunches are called staphylococciarrow_forward
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