Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 22, Problem 1AL
Summary Introduction
All the purple non-sulfur bacteria belong to α-proteobacteria, except Rhodocyclus. Rhodospirillum rubrum is a purple non-sulfur bacteria. It is a gram-negative, facultative anaerobe and is spiral shaped. R. rubrum is found in most of the natural aquatic environment such as lakes, ponds, streams, and also in sewage and mud.
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A series of auxotrophic mutants were isolated in Neurospora. Examination
of fungi containing these mutations revealed that they grew on minimal
medium to which various compounds (A, B, C, D) were added; growth
responses to each of the four compounds are presented in the following
table. Give the order of compounds A, B, C, and D in a biochemical
pathway. Outline a biochemical pathway that includes these four
compounds and indicate which step in the pathway is affected by each of
the mutations.
Compound
Mutation number
А в С
A
D
134
276
987
773
772
146
333
123
+ + I +
Researchers isolated a yeast phosphofructokinase (PFK) mutant in which a serine at the fructose-2,6-bisphosphate binding site was replaced with an aspartate residue. The amino acid substitution completely abolished the binding of fructose-2,6-bisphosphate to PFK. There was a dramatic decline in glucose consumption and ethanol production in the mutant compared to control yeast. Why can't the mutant PFK bind with fructose-2,6-bisphosphate?
Assume that a series of compounds has been discovered in Neurospora. Compounds A–F appear to be intermediates in a biochemical pathway. Conversion of one intermediate to the next is controlled by enzymes that are encoded by genes. Several mutations in these genes have been identified and Neurospora strains 1–4 each contain a single mutation. Strains 1–4 are grown on minimal media supplemented with one of the compounds A–F. The ability of each strain to grow when supplemented with different compounds is shown in the table (+ = growth; o = no growth).
Which biochemical pathway fits the data presented?
Media Supplement
Strain
A
B
C
D
E
F
1
o
o
o
+
+
+
2
o
o
o
o
+
+
3
o
o
o
o
+
o
4
o
o
+
+
+
+
A) A → B → C → D → E → F
B) A → B → C → F → D → E
C) F → B → C → D → A → E
D) A → B → C → D → F → E
E) A → B → F → E → C → D
Chapter 22 Solutions
Prescott's Microbiology
Ch. 22.1 - What would Rhodospirillum rubrum use for carbon...Ch. 22.1 - How are bacterial cysts different from the...Ch. 22.1 - If Caulobacter bacteria were grown in a...Ch. 22.1 - What steps are also found in the TCA cycle? What...Ch. 22.1 - Why do nitrifying microbes possess intracellular...Ch. 22.1 - Prob. 6MICh. 22.1 - Prob. 1CCCh. 22.1 - Prob. 2CCCh. 22.1 - Prob. 3CCCh. 22.1 - Compare and contrast the life cycle of members of...
Ch. 22.1 - Prob. 5CCCh. 22.1 - Prob. 6CCCh. 22.1 - Prob. 7CCCh. 22.1 - Prob. 8CCCh. 22.2 - MICRO INQUIRY Suggest why N. europaea has PTS...Ch. 22.2 - MICRO INQUIRY Identify the reactions that...Ch. 22.2 - Prob. 1CCCh. 22.2 - Prob. 2CCCh. 22.2 - Prob. 3CCCh. 22.2 - How do colorless sulfur bacteria obtain energy by...Ch. 22.2 - Prob. 5CCCh. 22.3 - Prob. 1MICh. 22.3 - Prob. 2MICh. 22.3 - Prob. 3MICh. 22.3 - Prob. 1CCCh. 22.3 - Prob. 2CCCh. 22.3 - Prob. 3CCCh. 22.3 - Prob. 4CCCh. 22.3 - Prob. 5CCCh. 22.3 - Prob. 6CCCh. 22.3 - Prob. 7CCCh. 22.4 - Prob. 1MICh. 22.4 - What general features are common to most...Ch. 22.4 - Prob. 2CCCh. 22.4 - Prob. 3CCCh. 22.4 - Characterize Bdellovibrio spp. and outline the...Ch. 22.4 - Compare the growth of a myxobacterium in...Ch. 22.5 - What property unifies -proteobacteria?Ch. 22.5 - Prob. 2CCCh. 22.5 - Prob. 3CCCh. 22 - Prob. 1RCCh. 22 - Prob. 2RCCh. 22 - Prob. 3RCCh. 22 - Prob. 4RCCh. 22 - Prob. 5RCCh. 22 - Prob. 6RCCh. 22 - Prob. 7RCCh. 22 - Prob. 8RCCh. 22 - Prob. 9RCCh. 22 - Prob. 1ALCh. 22 - Prob. 2ALCh. 22 - Prob. 3ALCh. 22 - Prob. 4ALCh. 22 - Prob. 5ALCh. 22 - Prob. 6ALCh. 22 - Prob. 7ALCh. 22 - Prob. 8ALCh. 22 - Prob. 9AL
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