Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 2, Problem 2.5CC
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CONCEPT CHECK 2.5
TMV is an RNA-containing virus. Mixing RNA from TMV strain A with coat protein from strain B results in infectious virus particles. How were these particles formed without outside information? Would these hybrid viruses infect the normal host of strain A or strain B?
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Results Labeling HIV-1 Capsids with a GFP Fluid Phase Marker. To determine whether viral cores in infected cells retained their integrity, we utilized a previously described HIV-1 vector in which GFP was inserted between matrix (MA) and CA (pNL4-3 Gag-iGFP) (35) (Fig. 1A). Proteolytic processing during virion maturation releases GFP from the Gag precursor, some of which remains trapped inside the capsid and is released upon loss of core integrity (Fig. 1B). We also utilized a previously described RNA stem-loop system in which 18 copies of BglG stem loops (BglSLs) were engineered into the HIV-1 genome in place of vif/vpr to facilitate the detection of nascent HIV-1 genomic RNA at the HIV-1 transcription site (Fig. 1A). This system can be used to identify the capsids that lead to productive infection and determine the nuclear location of integration (25). Virions were produced by cotransfection of an HIV-1 vector expressing Gag-iGFP and a vector expressing wild-type…
Question:-
what is the basic functions of a capsid, protomer, capsomere, and spikes in the viral infection and replication cycle?
QUESTION:
Explain the processes of the following experiments: Gel Electrophoresis, Plasmid Isolation, and Plasmid Injection into C. Elegans nematode. and why we are doing these experiments, what is the major outcome, and what can be achieved by applying these techniques in medicine?
Chapter 2 Solutions
Becker's World of the Cell (9th Edition)
Ch. 2 - What properties of the carbon atom make it...Ch. 2 - QUESTION: Compare the flights of the ionized...Ch. 2 - How would the properties of water change if the...Ch. 2 - Why is the amphipathic nature of membrane...Ch. 2 - Problem Set CONCEPT CHECK 2.4 What characteristics...Ch. 2 - Problem Set CONCEPT CHECK 2.5 TMV is an...Ch. 2 - The Fitness of Carbon. Each of the properties that...Ch. 2 - The Fitness of Water. For each of these statements...Ch. 2 - Wrong Again. For each of the following false...Ch. 2 - Prob. 2.4PS
Ch. 2 - Drug Targeting. Your company has developed a new...Ch. 2 - Its All About Membranes. Answer each of the...Ch. 2 - The Polarity of Water. Defend the assertion that...Ch. 2 - QUANTITATIVE Bond Energies. A single covalent bond...Ch. 2 - TMV Assembly. Each of these statements is an...Ch. 2 - Mars Is Alive? Imagine this futuristic scenario:...
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What Is A Virus ? ; Author: Peekaboo Kidz;https://www.youtube.com/watch?v=YS7vsBgWszI;License: Standard YouTube License, CC-BY