Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Textbook Question
Chapter 2, Problem 1Q
QUESTION: Compare the flights of the ionized molecular fragments whose paths are shown in blue and red in Figure 2A-1. Which fragment has the higher m/z ratio? What factors might explain why the deflection of that fragment differs from the other fragment?
Figure 2A-1 A Mass Spectrometer.
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Essay question: Compare the 3-D structure of proteins with the 3-D structure of DNA
Figure 3:
●
●
●
●
●
●
KDa
●
97.4
66.2
45.0
●
31.0-
21.5
14.4
S-1
p-1
S-2
2-0
This figure was generated by centrifuging a pura sample of protein, removing the supernatant,
and resuspending the pellet in the same volume as the supernatant to allow direct comparison.
The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via
normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The
text or number after the dash represents a different condition. For example, s-1 and p-1 are the
supernatant and pellet samples under condition 1. It is not shown, but under wild-type
conditions, essentially all of the protein is found in the supernatant.
S-3
● What does the intensity of each band represent?
● Would you find soluble protein in the supernatant or pellet? Why?
Would you find aggregated protein in the supernatant or pellet? Why?
For each condition (there are 5 different conditions), is there a higher percentage of the
total protein…
paper chromatography:
1.If the Rf of blue was 0.81, Rf of yellow was 0.68, and Rf of red was 0.64, then which color would be the heaviest? which one would be the lightest?
and why?
Gel electrophoresis:
2.Given that well 1 represented protein from a dog (Rf=0.45), well 2 from a wolf (Rf=0.73) and well 3 from a fox (Rf=0.76), and the unknown well represented protein from a "chupacabra". what animal would say the "chupacabra" is closely related to?
3.which one would be the heaviest and lightest in given above electrophoresis? and why?
Chapter 2 Solutions
Becker's World of the Cell (9th Edition)
Ch. 2 - What properties of the carbon atom make it...Ch. 2 - QUESTION: Compare the flights of the ionized...Ch. 2 - How would the properties of water change if the...Ch. 2 - Why is the amphipathic nature of membrane...Ch. 2 - Problem Set CONCEPT CHECK 2.4 What characteristics...Ch. 2 - Problem Set CONCEPT CHECK 2.5 TMV is an...Ch. 2 - The Fitness of Carbon. Each of the properties that...Ch. 2 - The Fitness of Water. For each of these statements...Ch. 2 - Wrong Again. For each of the following false...Ch. 2 - Prob. 2.4PS
Ch. 2 - Drug Targeting. Your company has developed a new...Ch. 2 - Its All About Membranes. Answer each of the...Ch. 2 - The Polarity of Water. Defend the assertion that...Ch. 2 - QUANTITATIVE Bond Energies. A single covalent bond...Ch. 2 - TMV Assembly. Each of these statements is an...Ch. 2 - Mars Is Alive? Imagine this futuristic scenario:...
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