Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 19.5, Problem 1CS
Gene Transfer Between Bacteria
Core Skill: Connections Look back at Figures 11.1 and 11.2. How did the phenomenon of transformation allow researchers to demonstrate that DNA is the genetic material?
Figure 11.1 Griffith’s experiments showing that genetic material can be transferred from one bacterium to another. Note: To determine if a mouse’s blood contained live bacteria, a sample of blood was also applied to solid growth media. (This part of the procedure is not shown.) For steps 1 and 4, smooth bacterial colonies were observed. For step 2, no bacterial colonies were observed because the type R cells were killed by the immune system of the mouse.
Figure 11.2 The Avery, MacLeod, and McCarty experiments that identified DNA as Griffith’s transformation principle—the genetic material.
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TOPIC: PCR and Gene Cloning Basics
Question: What are 2 possible roles of CaCl2 in the transformation process?
Q21:
Polymerase Chain Reaction (PCR) (Links to an external site.) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus (Links to an external site.) to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR.
If a researcher has a DNA sample with a concentration of 0.2 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 2.3 nanograms per microliter?
Report your answer to two decimal places using standard decimal notation (e.g. 0.5, not 5.0e-1)
AGAROSE GEL ELECTROPHORESIS PRELAB
ANSWER ALL QUESTIONS
1. Type of nucleotide is one of the factors that influence electrophoretic mobility.
a) True
b) False
2. Electrophoresis is used for DNA separation and not proteins.
a) True
b) False
3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis.
a)True
b)False
4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator.
a) True
b) False
5. During electrophoresis, DNA fragments collect at the Cathode.
a) True
b) False
Chapter 19 Solutions
Biology
Ch. 19.1 - Prob. 1CSCh. 19.2 - Prob. 1CSCh. 19.2 - Viral Reproductive Cycles Concept Check: From the...Ch. 19.4 - Genetic Properties of Bacteria Core Skill:...Ch. 19.4 - Prob. 1CCCh. 19.4 - Genetic Properties of Bacteria Concept Check:...Ch. 19.4 - Prob. 3CCCh. 19.5 - Prob. 1CCCh. 19.5 - Prob. 1EQCh. 19.5 - Prob. 2EQ
Ch. 19.5 - Gene Transfer Between Bacteria CoreSKILL The gene...Ch. 19.5 - Prob. 2CCCh. 19.5 - Gene Transfer Between Bacteria Core Skill:...Ch. 19.5 - Gene Transfer Between Bacteria Concept Check: Is...Ch. 19 - Prob. 1TYCh. 19 - The characteristics of viral genomes show many...Ch. 19 - During viral infection, attachment is usually...Ch. 19 - Prob. 4TYCh. 19 - Prob. 5TYCh. 19 - Prob. 6TYCh. 19 - Prob. 7TYCh. 19 - Prob. 8TYCh. 19 - Prob. 9TYCh. 19 - Prob. 10TYCh. 19 - How are viruses similar to living cells, and how...Ch. 19 - Prob. 2CQCh. 19 - Prob. 3CQCh. 19 - Prob. 1COQCh. 19 - Prob. 2COQ
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