Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 19.4, Problem 2CC
Genetic Properties of Bacteria
Concept Check: Describe the similarities and differences between a bacterial chromosome and a plasmid.
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Conjugation: Diagram the process of conjugation in bacterial cells (using F plasmid transfer):a. In you diagram, label the parts of both the donor and recipient cell, draw an arrow, then show the resulting cells and their resulting phenotypes. Label any other special features needed for this process.
Genome for C. diphtheriae have about 2,500,000 nucleotides, 87% of them are coding. This ingle circular chromosome contains 2,389 genes from which 2,272 proteins are coded. It does not contain any plasmids. The genome contains Pathogenicity Islands (PAIs), which C. diphtheriae has 13. What is a PAI and what are their characteristics?
art B-Processes occurring at a bacterial replication fork
The diagram below shows a bacterial replication fork and its principal proteins.
Drag the labels to their appropriate locations in the diagram to describe the name or function of each structure. Use pink labels for the pink targets and blue labels for the blue targ
Replaces RNA primers
with DNA nucleotides.
Catalyzes
phosphodiester bond
formation, joining
DNA fragments.
Leading
strand
Breaks hydrogen
bonds, unwinding
DNA double helix.
Synthesizes DNA 5' to
3 on leading and
lagging strands.
Lagging
strand
Coats single-stranded
DNA, preventing
duplex formation.
Relaxes supercoiled
DINA.
Synthesizes RNA
primers on leading
and lagging strands.
e
Overall direction
of synthesis
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Chapter 19 Solutions
Biology
Ch. 19.1 - Prob. 1CSCh. 19.2 - Prob. 1CSCh. 19.2 - Viral Reproductive Cycles Concept Check: From the...Ch. 19.4 - Genetic Properties of Bacteria Core Skill:...Ch. 19.4 - Prob. 1CCCh. 19.4 - Genetic Properties of Bacteria Concept Check:...Ch. 19.4 - Prob. 3CCCh. 19.5 - Prob. 1CCCh. 19.5 - Prob. 1EQCh. 19.5 - Prob. 2EQ
Ch. 19.5 - Gene Transfer Between Bacteria CoreSKILL The gene...Ch. 19.5 - Prob. 2CCCh. 19.5 - Gene Transfer Between Bacteria Core Skill:...Ch. 19.5 - Gene Transfer Between Bacteria Concept Check: Is...Ch. 19 - Prob. 1TYCh. 19 - The characteristics of viral genomes show many...Ch. 19 - During viral infection, attachment is usually...Ch. 19 - Prob. 4TYCh. 19 - Prob. 5TYCh. 19 - Prob. 6TYCh. 19 - Prob. 7TYCh. 19 - Prob. 8TYCh. 19 - Prob. 9TYCh. 19 - Prob. 10TYCh. 19 - How are viruses similar to living cells, and how...Ch. 19 - Prob. 2CQCh. 19 - Prob. 3CQCh. 19 - Prob. 1COQCh. 19 - Prob. 2COQ
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- Match. This is a diagram showing..? The structure identified by A The structure identified by B 1. plasmid 2. normal bacterial cell division 3. mRNA 4. horizontal gene transfer 5. Bacterial DNA in a bacteriophage 6. a virus such as SARS-CoV-2arrow_forwardYeast artificial chromosome how the synthesised?arrow_forwardhelp me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circulararrow_forward
- Small extrachromosomal rings of DNA, often contain genes that give bacterial cells genetic advantages: * none is correct O chromatin Onucleoplasm nucleoid plasmidsarrow_forwardQUESTION: what difficulty will you experience if you do genetic manipulation to streptomyces spp. and how can this difficulty overcome ?? how could you modulate the gene expression for improving the productivity of an antibiotic produced by the streptomyces strain ? discuss with diagramarrow_forwardSCi 7. Superhelical Density Bacteriophage A infects E. coli by wi integrating its DNA into the bacterial chromosome. The suc- cess of this recombination depends on the topology of the E. coli DNA. When the superhelical density (ơ) of the E. coli DNA is greater than -0.045, the probability of integration is <20%; when o is less than -0.06, the probability is –70%. the at lor 10 sic Plasmid DNA isolated from an E. coli culture is found to have is a length of 13,800 bp and an Lk of 1,222. Calculate o for this DNA and predict the likelihood that bacteriophage A will be 14 able to infect this culture. sisarrow_forward
- Activity 7. Build Me Up Direction: Construct a cladogram in the template provided using the data provided in the table below.arrow_forwardANALYSIS 1: Given the following sequence of bases present in the DNA coding strand, what will be the sequence on the template strand, on the MRNA strand and on the peptide? Show the sequence of amino acids if A is inserted between C-6 and T-7. (5') CTT-AGC-TGG-CCC... (3') Key-in your answers in this manner: template strand (3') XXX-XXX-XXX-XXX (3') MRNA strand (5') XXX-XXX-XXX-XXX (3) Wild-type peptide AA1-AA2-AA3-AA4 Mutant peptide AA1-AA2-AA3-AA4 2 buse n codon A. Phe Phe Sir Lni tase ne uopes n sarrow_forwardSensors detect the flash of light. DNA polymerase Unused deoxyribonucleotides are cleaved by apyrase. ATP is consumed by luciferase and light is emitted. AMP and PP, are converted into ATP by sulfurylase. Template strand Growing strand 3' TAGGCCTACACTTACGCGAATGT 5' 5' ATCCGGAT 3' dGTP dNTPs dNDPs dNMPs + P₁ PP₁ ATP [1]arrow_forward
- Give typing answer with explanation and conclusion 1. Which one of the following sequences best describes the flow of information from a gene to synthesis of a cellular component? A) RNA → DNA → RNA → protein B) DNA → RNA → protein C) DNA → amino acid → RNA → protein D) DNA → tRNA → mRNA → protein E) protein → RNA → DNAarrow_forwardation plasmids? 9. Predict and explain the effect of each of the following conditions on the Tm of native DNA. Will the Tm be raised or lowered relative to the DNA in Tris-HCl buffer, pH 7.5? (a) Measure Tm in pH 12 buffer. (b) Measure Tm in distilled water. (c) Measure Tm in 50% methanol water. (d) Measure Tm in standard Tris-HCI, pH 7.5 buffer solution containing SDS.arrow_forwardApplication/ Analysis Explain how the anti-parallel structure of DNA predicts its replication mechanism. Identify the major and minor groove of DNA and explain why they are there. Differentiate between semiconservative, conservative, and dispersive replication. Interpret a diagram of a bi-directional replication fork and correctly determine strand polarity and fork direction.arrow_forward
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