Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 18, Problem 71TQ
Summary Introduction
To review:
The way through which activity of reverse transcriptase results in amplification of the genes.
Introduction:
Gene amplification is the process through which a gene is amplified either in vivo or in vitro. In in vitro gene amplification, DNA (deoxyribonucleic acid) polymerase, single-strand binding proteins, DNA ligase, topoisomerases, and deoxyribonucleotides are required.
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QUESTION NO. 1
Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. The study of such patients has enhanced our knowledge of DNA repair because XP is caused by defective DNA repair nucleotide excision repair. (A variant, XP-V, is deficient in postreplication repair.) In nucleotide excision repair
A. removal of the damaged bases occurs on only one strand of the DNA.
B. only thymine dimers generated by UV light can be removed .
C. the excision nuclease is an exonuclease.
D. a single multifunctional enzyme carries out the repair process.
E. only the damaged nucleotides are removed.
QUESTION NO.2
Homologous recombination:
A. occurs only between two segments from the same DNA molecule.
B. requires that a specific DNA sequence be present.
C. requires one of the duplexes undergoing recombination be nicked in both strands.
D. involves a…
The eukaryotic mRNA is protected from degradation by a 3′ _____________.
QUESTION 19
Place the following steps in the expression of the lac operon in the order in which each occurs for the first time after a cell is induced.
v A phosphodiester bond is formed between two ribonucleotides.
- A peptide bond is formed between the first two amino acids in beta-galactosidase (lac Z).
Sigma protein binds to a specific DNA sequence.
RNA polymerase ends transcription of the lacA gene.
v A protein leaves an operator DNA sequence.
v A ribosomal RNA binds to a complementary sequence in mRNA.
Chapter 18 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 18 - Prob. 1QCh. 18 - Prob. 2QCh. 18 - Prob. 3QCh. 18 - Prob. 4QCh. 18 - Prob. 5QCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQ
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