Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 17, Problem 6CHI
Zymomonas mobilis is a Gram-negative bacterium that is used to make ethanol on an industrial scale. There is great interest in enhancing and expanding this microbe industrial uses. This prompted a group at the University of Hong Kong to develop a new E. coli–Z. mobilis shu vector. To do so, they started with a plasmid naturally found in some Z. mobilis strains. What genes and gene elements might the researchers need to add in order to construct a successful shuttle plasmid? What genetic elements do you think were added to obtain high-level expression of heterologous genes? Finally, how do you think they determined the copy number of the resulting shuttle vector in E. coli and in Z. mobilis?
Read the original paper: So, L. Y., et al., 2014. pZM Derived shuttle vectors for heterologous protein express and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis. BMC Microbiol. 14:68
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Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Why might two DNA fragments inadvertently be...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - If a linear piece of DNA is cut with a restriction...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - You want to visualize a digested plasmid that...Ch. 17.1 - What is the purpose of Southern blotting? How is a...Ch. 17.2 - Why, after three cycles, are the vast majority of...
Ch. 17.3 - Which plasmid is a shuttle vector? Why?Ch. 17.3 - What would you conclude if you obtained only blue...Ch. 17.3 - In what ways does the BAC shown here differ from...Ch. 17.3 - Briefly describe the polymerase chain reaction....Ch. 17.3 - Why is PCR used to detect infectious agents that...Ch. 17.3 - How would you use PCR to measure the concentration...Ch. 17.3 - Why is it possible to visualize a PCR product on...Ch. 17.3 - You want to clone a 6,000 bp DNA fragment in E...Ch. 17.5 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.6 - What special considerations are necessary if one...Ch. 17.6 - Prob. 1RIACh. 17.6 - Explain the selection for antibiotic resistance...Ch. 17.6 - Prob. 3RIACh. 17.6 - Prob. 4RIACh. 17.6 - Prob. 5RIACh. 17.6 - You are studying chemotaxis proteins in a newly...Ch. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - You are performing a PCR to amplify a gene...Ch. 17 - Prob. 2CHICh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5CHICh. 17 - Zymomonas mobilis is a Gram-negative bacterium...
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