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Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 17.1, Problem 2RIA
What is cDNA? Why is it necessary to generate cDNA before cloning and expressing a eukaryotic gene in a bacterium?
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calculate the questions showing the solution including variables,unit and equations all the questiosn below using the data.show solving and answer
a) B1,
b) B2,
c) hybrid rate constant (1)
d) hybrid rate constant (2)
e) t1/2,dist
t1/2,absorb
f) t1/2,elim
k) apparent central compartment volume (V1,app)
p) total AUC (using short cut method)
apparent volume of distribution based on AUC (VAUC,app)
apparent clearance (CLapp)
absolute bioavailabilty of oral route ( AUCiv =116ml)
Please help me to draw this by hand. In as much detail as possible, hand draw a schematic diagram of the hypothalamic-pituitary-gonad (HPG) axis in the human female. Be sure to include all the relevant structures and hormones. You must define all abbreviations the first time you use them. Please include (and explain) the feedback loops.
Please refer below
Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Why might two DNA fragments inadvertently be...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - If a linear piece of DNA is cut with a restriction...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - You want to visualize a digested plasmid that...Ch. 17.1 - What is the purpose of Southern blotting? How is a...Ch. 17.2 - Why, after three cycles, are the vast majority of...
Ch. 17.3 - Which plasmid is a shuttle vector? Why?Ch. 17.3 - What would you conclude if you obtained only blue...Ch. 17.3 - In what ways does the BAC shown here differ from...Ch. 17.3 - Briefly describe the polymerase chain reaction....Ch. 17.3 - Why is PCR used to detect infectious agents that...Ch. 17.3 - How would you use PCR to measure the concentration...Ch. 17.3 - Why is it possible to visualize a PCR product on...Ch. 17.3 - You want to clone a 6,000 bp DNA fragment in E...Ch. 17.5 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.6 - What special considerations are necessary if one...Ch. 17.6 - Prob. 1RIACh. 17.6 - Explain the selection for antibiotic resistance...Ch. 17.6 - Prob. 3RIACh. 17.6 - Prob. 4RIACh. 17.6 - Prob. 5RIACh. 17.6 - You are studying chemotaxis proteins in a newly...Ch. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - You are performing a PCR to amplify a gene...Ch. 17 - Prob. 2CHICh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5CHICh. 17 - Zymomonas mobilis is a Gram-negative bacterium...
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- If four babies are born on a given day What is the chance all four will be girls? Use genetics lawsarrow_forwardExplain each punnet square results (genotypes and probabilities)arrow_forwardGive the terminal regression line equation and R or R2 value: Give the x axis (name and units, if any) of the terminal line: Give the y axis (name and units, if any) of the terminal line: Give the first residual regression line equation and R or R2 value: Give the x axis (name and units, if any) of the first residual line : Give the y axis (name and units, if any) of the first residual line: Give the second residual regression line equation and R or R2 value: Give the x axis (name and units, if any) of the second residual line: Give the y axis (name and units, if any) of the second residual line: a) B1 Solution b) B2 c)hybrid rate constant (λ1) d)hybrid rate constant (λ2) e) ka f) t1/2,absorb g) t1/2, dist h) t1/2, elim i)apparent central compartment volume (V1,app) j) total AUC (short cut method) k) apparent volume of distribution based on AUC (VAUC,app) l)apparent clearance (CLapp) m) absolute bioavailability of oral route (need AUCiv…arrow_forward
- You inject morpholino oligonucleotides that inhibit the translation of follistatin, chordin, and noggin (FCN) at the 1 cell stage of a frog embryo. What is the effect on neurulation in the resulting embryo? Propose an experiment that would rescue an embryo injected with FCN morpholinos.arrow_forwardParticipants will be asked to create a meme regarding a topic relevant to the department of Geography, Geomatics, and Environmental Studies. Prompt: Using an online art style of your choice, please make a meme related to the study of Geography, Environment, or Geomatics.arrow_forwardPlekhg5 functions in bottle cell formation, and Shroom3 functions in neural plate closure, yet the phenotype of injecting mRNA of each into the animal pole of a fertilized egg is very similar. What is the phenotype, and why is the phenotype so similar? Is the phenotype going to be that there is a disruption of the formation of the neural tube for both of these because bottle cell formation is necessary for the neural plate to fold in forming the neural tube and Shroom3 is further needed to close the neural plate? So since both Plekhg5 and Shroom3 are used in forming the neural tube, injecting the mRNA will just lead to neural tube deformity?arrow_forward
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