BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 16, Problem 3IQ
Summary Introduction

To determine: The reasons for polyubiquitination of polypeptides or proteins

Introduction: The process of degradation of protein, which triggered by the marking of ubiquitin, is known as polyubiquitination. Polyubiquitination is an essential pathway because it removes protein molecules that are old, unused, or improperly folded.

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8. Multiple choice. Which of the following is not a method that can be used to determine the 3° structure of proteins? (2 pts) a) Protein X-ray crystallography b)) Circular dichroism c) Cryogenic electron microscopy d) Protein NMR 9. Multiple choice. Approximately what overall yield can you expect from the solid phase peptide synthesis (SPPS) of a "10-mer" (a 10 amino acid peptide), if the yield from each step is 85%? (2 pts) 23% b) 20% c) 85% d) 71% 10. Multiple choice. Which protein purification method is best for an experiment in which you want to purify a monomeric form of a protein away from a tetrameric form? (2 ptrs) (-His a) Affinity chromatography S b) High pressure liquid chromatography c) lon exchange chrommatography d) Size exclusion chromatography 2. I1. Short answer. What is the Edman degradation experiment useful for? (3 pts) purdinate an for prry structers steps .
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