BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 16, Problem 2S
Examples of positive and negative control of transcription can be found in the regulation of expression of the bacterial operons lac and trp. Use these two operon systems to describe the difference between positive and negative regulation.
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Describe how the components of the trp operon system are affected by a mutation in trpC?
Why is it that once enviornmental tryptophan is inserted into the cell, only the trp repressor is activated (turned on)?
Under low tryptophanyl-tRNA (tRNAtrp), we expect the trp operon to be expressed because:
Question 13 options:
Sections 1 and 2 of the leader form a hairpin that acts as a transcriptional terminator.
Sections 2 and 3 of the leader form a hairpin that prevents the formation of a transcriptional terminator.
Sections 1 and 2 of the leader form a hairpin that prevents the formation of a transcriptional terminator.
Sections 2 and 3 of the leader form a hairpin that acts as a transcriptional terminator.
Sections 3 and 4 of the leader form a hairpin that acts as a transcriptional terminator.
For the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor.
Q. Negative control in a repressible operon
Chapter 16 Solutions
BIOLOGY
Ch. 16.1 - Prob. 1LOCh. 16.1 - Prob. 2LOCh. 16.1 - Prob. 3LOCh. 16.2 - Explain how proteins can interact with base-pairs...Ch. 16.2 - Prob. 2LOCh. 16.3 - Prob. 1LOCh. 16.3 - Prob. 2LOCh. 16.3 - Explain control of gene expression in the trp...Ch. 16.4 - Prob. 1LOCh. 16.4 - Prob. 2LO
Ch. 16.4 - Prob. 3LOCh. 16.5 - Describe at least two kinds of epigenetic mark.Ch. 16.5 - Explain the function of chromatin-remodeling...Ch. 16.6 - Prob. 1LOCh. 16.6 - Prob. 2LOCh. 16.7 - Prob. 1LOCh. 16.7 - Prob. 2LOCh. 16 - Prob. 1DACh. 16 - What advantage might a bacterium gain by linking...Ch. 16 - Prob. 2IQCh. 16 - Prob. 3IQCh. 16 - In prokaryotes, control of gene expression usually...Ch. 16 - Prob. 2UCh. 16 - Prob. 3UCh. 16 - The lac operon is controlled by two main proteins....Ch. 16 - In eukaryotes, binding of RNA polymerase to a...Ch. 16 - In eukaryotes, the regulation of gene expression...Ch. 16 - In the trp operon, the repressor binds to DNA a....Ch. 16 - Prob. 1ACh. 16 - Specific transcription factors in eukaryotes...Ch. 16 - Repression in the trp operon and induction in the...Ch. 16 - Regulation by small RNAs and alternative splicing...Ch. 16 - Eukaryotic mRNAs differ from prokaryotic mRNAs in...Ch. 16 - In the cell cycle, cyclin proteins are produced in...Ch. 16 - A mechanism of control in E. coli not discussed in...Ch. 16 - You have isolated a series of mutants affecting...Ch. 16 - Examples of positive and negative control of...Ch. 16 - What forms of eukaryotic control of gene...Ch. 16 - The number and type of proteins found in a cell...
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- When referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, transcription termination at the attenuator region is likely the ribosomes stall during translation of the attenuator region translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels tryptophan inactivates the repressor proteinarrow_forwardExplore the differences between positive inducible, positive repressible, negative inducible, and negative repressible regulation. For the example portion choose between only these 4 options: glucose regulating lac, lactose regulating lac, and tryptophan regulating trp, none Positive Inducible Does binding of the regulator stimulate or inhibit transcription? In the absence of the signal, will the operon be active or inactive? Example of this type of regulation that has been covered in class positive repressible Does binding of the regulator stimulate or inhibit transcription? In the absence of the signal, will the operon be active or inactive? Example of this type of regulation that has been covered in class negative inducible Does binding of the regulator stimulate or inhibit transcription? In the absence of the signal, will the operon be active or inactive? Example of this type of regulation that has been covered in class…arrow_forwardFor the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor. Q. Negative control in an inducible operonarrow_forward
- Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. b) Predict what will happen if trp repressor lose its allosteric effect.arrow_forwardWhen CAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the protein, which results in binding to the CAP- binding site on the DNA and molecule is known as a(n) of the lac operon. This conformational change induced by the binding of another transcription activation; allosteric effect transcription repression; binding effect translation; distortion effect mutation; interactive effect attenuation; compression effect O O O Oarrow_forwardIn the regulation of the tryophan operon in bacteria, the co-occurence of transcription and translation plays a key role regulating full activation of operon expression. Diagram and explain how during the conditions of high tryptophan concentration this system regulates the expression of genes in the operon.arrow_forward
- Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor. List and explain the effect of such a mutation on the expression of the structural gene for lactose in E.coli?arrow_forwardWhat would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)arrow_forwardTryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. c) Review the region that is responsible for trp attenuation during low trp level.arrow_forward
- As diagrammed below, some amino acid biosynthetic operons have stem loop structures in the mRNA. These structures play a role in the regulation of the operon by which of the following mechanisms? Translation stop codon- Attenuator loop. mRNA 5' 3' DNA attenuator trpE O Catabolite repressor protein can bind and prevent the hairpins from forming thus allowing translation to occur. O The leader sequence shown contains codons for the amino acid. When these are low, the ribosome stalls, allowing a hairpin between the two middle regions to form so that the rest of the message can be translated. In the presence of large amounts of the amino acid, the hairpin will wind around the amino acid and prevent further translation. O A repressor protein made of that amino acid will bind to the region and prevent translation when large amounts of the amino acid are present. O The uridine-rich region has codons for that amino acid; when present, this region will be a terminator region. The hairpins allow…arrow_forwardExplain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.arrow_forwardWhich of the following statements about the trp operon is false? If a mutation in region 2 prevents the formation of the 2 + 3 loop in the 5’UTR transcription levels will increase. The trp operon is an example of negative repressible operon. Formation of the anti-terminator in the 5’UTR of the trp operon occurs when trp levels are low. In the presence of high trp-tRNA, the genes of the trp operon are not expressed. The role of trp is to bind to the repressor protein and activate it.arrow_forward
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