The model for structure of mutated repressor in the presence and absence of tryptophan. Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE , trpD , trpC , trpB , and trpA genes.

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Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

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Enhancer RPA Gene A Use the diagram above, which depicts a chromosomal region less than 500,000 bp, to determine the expression of each gene under the scenarios specified in the table. Regulatory promoters are indicated by "RP". Genes A, B, and C have intermediate expression when no regulatory proteins are bound. Complete the table by choosing high, intermediate, or low to describe the expression level of each gene under each specified scenario (i and ii). No regulatory proteins bound (i) A repressor binds to RPA and an activator binds to RPC (ii) A regulatory protein binds at each of the activator, insulator, and silencer. Expression of A? Intermediate 1. [Select] 4. [Select] Insulator RPB Expression of B? 2. Gene B Intermediate 5. [Select] [Select] Silencer RPC Expression of C? 3. Gene C Intermediate 6. [Select] [Select]

E30. An electrophoretic mobility shift assay can be used to study the binding of proteins to a segment of DNA. In the experiment shown here, an EMSA was used to examine the requirements for the bind- ing of RNA polymerase II (from eukaryotic cells) to the promoter of a protein-encoding gene. The assembly of general transcription factors and RNA polymerase II at the core promoter is described in Chapter 12 (Figure 12.14). In this experiment, the segment of DNA containing a promoter sequence was 1100 bp in length. The fragment was mixed with various combinations of proteins and then subjected to an EMSA. Lane 1: No proteins added Lane 2: TFID Lane 3: TFIIB Lane 4: RNA polymerase I| Lane 5: TFID + TFIIB Lane 6: TFID + RNA 1 2 4 5 6 polymerase II| Lane 7: TFIID + TFIIB + RNA polymerase I| 1100 bp Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are able to bind to this DNA fragment by themselves. Which transcrip- tion factors (i.e., TFIID or TFIIB) are needed for the binding of…

Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator lac Y lac A DNA +1 1. What are structural genes? Are the lac structural genes transcribed in the absence of lactose? 2. What is the role of the promoter and operator sites in the operon? 3. Is the repressor protein bound to the operator site in the absence of lactose? In its absence? 4. Under what nutritional circumstances (high or low glucose) is CAP bound to cAMP? 5. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 6. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 7. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 8. Why is it adaptive for a bacterium to not…

Answer 2

Question

Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

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Answer 3

Question

Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

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Answer 4

Question

Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

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Answer 5

Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Answer 6

Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Answer 7

Chapter 14.2, Problem 2CS

Summary Introduction

To determine: The model for structure of mutated repressor in the presence and absence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Answer 8

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Answer 9

Summary Introduction

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Answer 10

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Answer 13

Ch. 14.1 - Prob. 1CC Ch. 14.2 - Which genes are under the control of the lac...Ch. 14.2 - With regard to regulatory proteins and small...Ch. 14.2 - What were the key observations made by Jacob,...Ch. 14.2 - CoreSKILL What was the eventual hypothesis...Ch. 14.2 - Prob. 3EQ Ch. 14.2 - Core Skill: Connections Look back at Fig 9.12....Ch. 14.2 - What are the advantages of having both an...Ch. 14.2 - Prob. 2CS Ch. 14.3 - Prob. 1CC

The model for structure of mutated repressor in the presence and absence of tryptophan. Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE , trpD , trpC , trpB , and trpA genes.

Solution Summary: The author explains how the trp operon encodes enzymes that are essential to make tryptophan amino acid.

Concept explainers

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To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

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Chapter 14 Solutions

The model for structure of mutated repressor in the presence and absence of tryptophan. Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE , trpD , trpC , trpB , and trpA genes.

Solution Summary: The author explains how the trp operon encodes enzymes that are essential to make tryptophan amino acid.

Concept explainers

Videos

To determine: Whether or not trp operon would be repressed in presence of tryptophan. Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.

Want to see the full answer?

Chapter 14 Solutions

To determine: Whether or not trp operon would be repressed in presence of tryptophan.

Introduction: The trp operon encodes enzymes that are very essential to make tryptophan amino acid. In this pathway, synthesis of tryptophan occurs with the help of trpE, trpD, trpC, trpB, and trpA genes.