The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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You are interested in studying resistance to heavy metals and have selected the yeast Saccharomyces cerevisea to conduct your studies. You have recovered a deletion mutant that does not tolerate high concentrations of zinc (grows poorly in zinc containing media ) and have designated the mutant pgz-1 (for poor growth in zinc ). (a) What is the advantage to the type of mutant used in this work? What class of mutagen was likely use to generate pgz-1? ( b) Do you expect the PGZ gene to be expressed in your mutant? Explain.

Five individual His- mutants (hisA, his B, hisC, hisD and his E) were isolated. The genes are all involved in the histidine biosynthetic pathway. A crossfeeding experiment was carried out to determine the order of the enzymes encoded by these genes. The result is shown (A, B, C, D and E represent the mutants hisA, his B, hisC, hisD and his E, respectively. Dark area indicates bacterial growth). From the result shown, what might be the histidine biosynthetic pathway (the order of enzymes)?

The DNA sequence of one strand of a gene from threeindependently isolated mutants is given here (5′ endsare at left). Using this information, what is the sequence of the wild-type gene in this region?mutant 1 ACCGTAATCGACTGGTAAACTTTGCGCGmutant 2 ACCGTAGTCGACCGGTAAACTTTGCGCGmutant 3 ACCGTAGTCGACTGGTTAACTTTGCGCG

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Question

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Answer 3

Question

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Answer 4

Question

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Expert Solution & Answer

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Answer 5

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Answer 6

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Answer 7

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Answer 8

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Answer 11

Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LO Ch. 14.3 - Prob. 1LO Ch. 14.3 - Prob. 2LO Ch. 14.4 - Prob. 1LO Ch. 14.4 - Prob. 2LO Ch. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.

The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Solution Summary: The author explains the phenotype of Dam mutants. The enzyme adenine methylase works in the 5’-GATC-3’ direction.

Concept explainers

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To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Want to see the full answer?

Chapter 14 Solutions

The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Solution Summary: The author explains the phenotype of Dam mutants. The enzyme adenine methylase works in the 5’-GATC-3’ direction.

Concept explainers

Videos

To determine: The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Want to see the full answer?

Chapter 14 Solutions

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.