BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 13.4, Problem 3LO
Summary Introduction

To determine: Map distances from the frequency of recombinants in test crosses.

Introduction: The genes located on the different chromosomes assort independently at the time of gamete formation and linked genes cannot assort independently.

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In the sample data from Sordaria shown in the picture: Count the total number of first and second segregations and calculate map units using equation below:   (recombinant refers to second division segregation and non-recombinants are first division segregations)
If 2 genes on the same chromosome have a recombination frequency of .01, what is the genetic distance from each other in map units?
Consider the first category of test-cross offspring shown in figure 8.2 (+b, LS). Consider also that the parents of the heterozygous female flies in the test cross had the following genotypes: bb, SS, and +, LL. A. What would be the physical phenotype of these flies? B. If PC was conducted with the DNA of one of these flies using the primers for the molecular marker, what would be the appearance of the bands on an electrophoresis gel with the PC products? C. If the gene for black body and the locus for the molecular marker (L long or S short) were unlinked, what proportion of the test-cross progeny would be black flies that are heterozygous for the molecular marker? What proportion would be flies with normal body color, which are homozygous for one form of the molecular marker? D. If the gene for black body and the locus for the molecular marker were linked, how would the proportion of flies be different?
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