Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 11.9, Problem 1CR
Q What is a merodiploid and how does an F′ plasmid yield a merodiploid?
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In what ways do plasmid differ from an episome?
1 (a) What do ApR, TcR, and ori on the pBR322 map represent and discuss there individual functions?
(b)Does the undigested plasmid show more than a single band when electrophoresed? Why?
(c)What other kinds of molecules, in addition to plasmid DNA would you expect to find in a sample of plasmid DNA extraction? (This is not a graded assignment)
What is the role of X-gal? What color will cells with the plasmid be in the absence of X-gal? Why?
Chapter 11 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 11.1 - Distinguish between a mutation and a mutant.Ch. 11.1 - Distinguish between screening and selection.Ch. 11.1 - Prob. 3MQCh. 11.1 - Write a one-sentence definition of the term...Ch. 11.2 - Do missense mutations occur in genes encoding...Ch. 11.2 - Why do frameshift mutations generally have more...Ch. 11.2 - Prob. 1CRCh. 11.3 - Why are suppressor tRNA mutations not lethal?Ch. 11.3 - Which class of mutation, missense or nonsense, is...Ch. 11.3 - What is the difference between same-site and...
Ch. 11.4 - Prob. 1MQCh. 11.4 - Prob. 2MQCh. 11.4 - Prob. 1CRCh. 11.5 - Which protein, found in virtually all cells,...Ch. 11.5 - Explain the fate of transferred chromosomal DNA if...Ch. 11.5 - Prob. 3MQCh. 11.5 - What are heteroduplex regions of DNA and what...Ch. 11.6 - During transformation a cell usually incorporates...Ch. 11.6 - In genetic transformation, what is meant by the...Ch. 11.6 - QExplain why recipient cells do not successfully...Ch. 11.7 - Prob. 1MQCh. 11.7 - What is the major difference between generalized...Ch. 11.7 - Why is phage conversion considered beneficial to...Ch. 11.7 - QExplain how a generalized transducing particle...Ch. 11.8 - In conjugation, how are donor and recipient cells...Ch. 11.8 - Explain how rolling circle DNA replication allows...Ch. 11.8 - QWhat is a sex pilus and which cell type, F or F+,...Ch. 11.9 - In conjugation involving the F plasmid of...Ch. 11.9 - Prob. 2MQCh. 11.9 - Prob. 3MQCh. 11.9 - QWhat is a merodiploid and how does an F plasmid...Ch. 11.10 - Why is it usually more difficult to select...Ch. 11.10 - Why do penicillins not kill species of Archaea?Ch. 11.10 - Explain one type of conjugation in Archaea and how...Ch. 11.11 - Prob. 1MQCh. 11.11 - What is the significance of the terminal inverted...Ch. 11.11 - How can transposons be used in bacterial genetics?Ch. 11.11 - Prob. 1CRCh. 11.12 - Why is the CRISPR system considered a prokaryotic...Ch. 11.12 - Prob. 2MQCh. 11.12 - QExplain why incoming DNA recognized by a short...Ch. 11 - A constitutive mutant is a strain that...Ch. 11 - Although a large number of mutagenic chemicals are...Ch. 11 - Why is it difficult in a single experiment to...Ch. 11 - Prob. 4AQ
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- What is true regarding the following cross between bacterial strains: F’ x F-? As the donor, F' will donate only the F plasmid to F- As the donor, F- will donate the F plasmid only to F' As the donor, F- will donate the F plasmid and a portion of the bacterial chromosome to F' As the donor, F' will donate the F plasmid and a portion of the bacterial chromosome to F-arrow_forwardDescribe the genetic makeup of a plasmid in an F’ cell, including the types and origins of genetic information contained in this plasmid, and the mechanism by which such a plasmid arises.arrow_forwardCalculate the linking number “L” for a 210 base pair closed circular plasmid with 4 negative supercoils. Assume the DNA remains entirely B-form. A) 10 B) 16 C) 24 D) -10 E) -4 Please solve and why is it B? Need answer in short and asap .arrow_forward
- Choose the statement below that is true about the characteristics of the bacterial fertility factor factor. The fertility factor carries genes responsible for uptake of naked DNA, and has several IS elements to assist plasmid integration into host cell's chromosome. O The fertility factor carries genes responsible for formation of bacteriophages, and has several IS elements to assist plasmid transfer through the sex pilus. The fertlity factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid integration into host cell's chromosome. The fertility factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid transfer through the sex pilus.arrow_forwardWhat is a mobilizable plasmid?arrow_forwardWhat is a merodiploid and how does an F¿ plasmidyield a merodiploid?arrow_forward
- Assume a 5250 base pair, closed circular plasmid with 10 negativesupercoils.(a) Calculate the values of twist (T), writhe (W), and linking number (L) for thisplasmid (use 10.5 base pairs/turn for B-DNA)(b) Your starting plasmid is now A-DNA instead of B-DNA. What is the linkingnumber required to have 10 negative supercoils in this plasmid? (R = 8.3145 J−1 K mol−1; 0 ◦C = 273.15 K; Faraday Constant , F =96485 J V−1 mol−1.)arrow_forwardWhat happens to YAC • Plasmids containing only origins of replication but no centromere or telomere?arrow_forwardWhat is the difference between a l insertion vector such aslgt11 and a l replacement vector? What is the advantage ofeach?arrow_forward
- What is the relationship between a PRR and a PAMP?arrow_forwardExplain the basis for the following statement: Transcription of two genes on a plasmid can occur without the concomitant action of a topoisomerase, but only if those two genes are oriented in opposite directions.arrow_forwardWhat are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?arrow_forward
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