Campbell Essential Biology (7th Edition)
7th Edition
ISBN: 9780134765037
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 11, Problem 17PS
The human body has a far greater variety of proteins than genes, highlighting the importance of alternative RNA splicing. Suppose you have samples of two types of adult cells from one person Design an experiment using microarrays to determine whether different gene expression is due to alternative RNA splicing.
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Northern blotting is used to determine the amount and size of a particular RNA transcribed in a given cell type. Alternative splicing produces mRNAs of different lengths of the same gene. In the following figure three probes were used: PSAT, PHGDH and Actin (house keeping gene). Discuss the results in detail.
Which tissue showed higher expression?
Why actin is used?
You are studying a rare form of brain cancer where the newly-discovered genes BR and AIN are known to play a role in the development of this cancer. You perform a RNA-seq analysis on a patient with this form of cancer and compare the resulting expression histogram to a healthy patient. You conclude that AIN is heavily upregulated in the cancer patient and that the cancer patient's mRNA for BR retains one of that gene's introns in the RNA-seq results. Based on this information, BR is likely a(n) _________ and AIN is likely a(n) _________ .
a.
oncogene; tumor-suppressor gene
b.
tumor-suppressor gene; tumor-suppressor gene
c.
oncogene; oncogene
d.
tumor-suppressor gene; viral gene
e.
tumor-suppressor gene; oncogene
Could quantitative PCR, which uses a DNA-binding dye, to show how many copies of the target DNA sequence could be used to quantify the amount of mRNA in a cell? Would you expect that a metabolically active tissue such as the liver would show more cDNA copies in such a method, compared to less metabolically active tissues such as skin cells?
One reason that the types and amounts of mRNAs are quantified in different tissue types is to compare which genes are activated and which are inactive. It used to be thought that any gene that was transcribed was automatically translated. The discovery of RNA-degrading systems shows that the real situation in cells is more complemented. Do you believe that a larger amount of mRNA of a given type, say for alpha hemoglobin in immature red blood cells is a reliable indicator that more alpha hemoglobin protein will be made in those cells?
Chapter 11 Solutions
Campbell Essential Biology (7th Edition)
Ch. 11 - Your bore cells, muscle cells, and skin cells look...Ch. 11 - A group of prokaryotic genes with related...Ch. 11 - The regulation of gene expression must be more...Ch. 11 - A eukaryotic gene was inserted into the DNA of a...Ch. 11 - How does DNA packing in chromosomes prevent gene...Ch. 11 - What evidence demonstrates that differentiated...Ch. 11 - The most common procedure for cloning an animal is...Ch. 11 - Prob. 8SQCh. 11 - Prob. 9SQCh. 11 - Prob. 10SQ
Ch. 11 - What is the difference between oncogenes and...Ch. 11 - Prob. 12SQCh. 11 - For each statement, identify which major theme is...Ch. 11 - For each statement, identify which major theme is...Ch. 11 - Prob. 15IMTCh. 11 - Study the depiction of the lac operon in Figure...Ch. 11 - The human body has a far greater variety of...Ch. 11 - Because a cat must have both orange and non-orange...Ch. 11 - Design a DNA microarray experiment that measures...Ch. 11 - Interpreting Data Review Figure 11.22 We can...Ch. 11 - A chemical called dioxin present in Agent Orange,...Ch. 11 - There are genetic tests for several types of...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- IS. Alternative splicing has been estimated to occur in more than 95% of multi-exon genes. Which of the following is not an evolutionary advantage of alternative splicing? Alternative splicing increases diversity without increasing genome size Different gene isoforms can be expressed in different tissues Alternative splicing creates shorter mRNA transcripts Different gene isoforms can be expressed during different stages of development.arrow_forwardThe pre-mRNA transcript and protein made by several mutant genes were examined. The results are given below. Determine where in the gene a likely mutation lies: the promoter region, exon, intron, cap on mRNA, or ribosome binding site. a. normal-length transcript, normal-length nonfunctional protein b. normal-length transcript, no protein made c. normal-length transcript, normal-length mRNA, short nonfunctional protein d. normal-length transcript, longer mRNA, shorter nonfunctional protein e. transcript never madearrow_forwardYou are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You take your DNA construct and place it into E. coli. You measure protein expression and notice that no protein is being made. Provide 1 reason why you don't see protein as it relates to transcription and how this problem could be resolved.arrow_forward
- Bacteria are often the preferred hosts for splicing in novel genes. For instance, the gene for human insulin was isolated and moved into a bacterium, which can now produce the much-needed chemical. Previously, type 1 diabetics had to rely on professionals that gathered insulin from human cadavers, cows, and pigs. In order for this feat of genetic engineering to occur, researchers had to start with an unspliced MRNA transcript for sinsulin. Agree/Disagree? Explain your response.arrow_forwardCreate a Venn diagram to compare and contrast the process of gene expression in Bacteria versus eukaryotes. Remember that “gene expression” can include any part of transcription or translation. Try to be as thorough as you can about what aspects of this process are similar between the two taxa, and what characteristics are distinct to only Bacteria or eukaryotes. Plase include a minimum of 15 items in the Venn diagram.arrow_forwardDuring experimental RNAi, how does the researcher affect expression of a target gene? Group of answer choices Inject custom, lab-made double-stranded RNA into the specimen that will be targeted by the cell's existing Dicer to create siRNA complementary to the target gene's transcript. Inject lab-made Dicer proteins into the specimen which will cut up the target gene's transcript. Inject custom, lab-made siRNAs complementary to the target gene's transcript.arrow_forward
- A molecular geneticist hopes to find a gene gene in human liver cells that codes for an important blood clotting protein. He knows that the nucleotides sequence of a small part of the gene is GTGGACTGACA. briefly explain how to obtain the desired genearrow_forwardDescribes a method known as Western blotting that can be used to detect a polypeptide that is translated from a particular mRNA. In this method, a particular polypeptide or protein is detected by an antibody that specifically recognizes a segment of its amino acid sequence. After the antibody binds to the polypeptide within a gel, a secondary antibody (which is labeled) is used to visualize the polypeptide as a dark band.For example, an antibody that recognizes α-galactosidase A couldbe used to specifically detect the amount of α-galactosidase A proteinon a gel. The enzyme α-galactosidase A is defective in individuals with Fabry disease, which shows an X-linked recessive pattern of inheritance. Amy, Nan, and Pete are siblings, and Pete has Fabry disease. Aileen, Jason, and Jerry are brothers and sister, and Jerry has Fabry disease. Amy, Nan, and Pete are not related to Aileen, Jason, and Jerry. Amy, Nan, and Aileen are concerned that they could be carriers of a defective…arrow_forwarda molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. he knows that the nucleotide sequence of a small part of the gene is gtggactgaca. briefly explain how to obtain the desired gene answerarrow_forward
- You are a research scientist working in genetic engineering. You create a piece of DNA that you want to express in E. coli, a prokaryote. This piece of DNA consists of a bacterial promoter, a ribosome binding sequence (RBS), a eukaryotic gene and a terminator sequence. Do you think that this piece of DNA would be expressed if placed into an E. coli cell that contains all the machinery needed for gene expression?arrow_forwardImagine that you and your colleagues are working in a lab to develop a protein synthesis system for a new type of synthetic cell. During your brainstorming sessions, you propose that polycistronic mRNA would be much more useful than mRNA that is only translated into one protein. This would allow for multiple proteins necessary for a particular function to be translated together. One of your colleagues says that is a good idea, but if you decide to go with polycistronic mRNA, you’d better make sure to use a prokaryotic translation system. Why might it be problematic to use a eukaryotic translation system with polycistronic mRNA? Mention three reasons:arrow_forwardConsider this list (below) of steps involved in transcription. These steps are out of order. TRANSCRIPTION: 1. mRNA travels through a nuclear pore and enters the cytoplasm 2. the mRNA polymerase attaches at the start of a specific gene 3. RNA polymerase reads the gene surface4. a transcription factor bonds to a promoter site5. DNA molecule is unwound 6. a complimentary mRNA is produced What is the correct order of this transcription?arrow_forward
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